Abstract: The Hippo signaling pathway is an evolutionarily conserved signaling pathway centered on the kinase cascade reaction that plays a key role in regulating organ size, tissue homeostasis, tissue regeneration, wound healing and tumorigenesis in mammals. The Hippo signaling pathway is stimulated by changes in cell density, external mechanical stress and/or other intrinsic and extrinsic signals that cause the core complex controlling the transcriptional co-activator Yes-associated protein (YAP) and transcriptional co-activator with PDZ binding motif protein to translocate into the nucleus, thereby regulating various biological effects. In addition, YAP, as a major effector molecule of Hippo signaling pathway, is aberrantly expressed in many tumors and is closely related to tumorigenesis and development, including tumor cell proliferation and apoptosis, tumor metastasis and drug resistance, etc. The effect of Hippo signaling pathway on tumor cell radiation effect is mainly reflected in the effect of YAP on tumor cell radiosensitivity and radiation resistance. The author reviews the molecular types regulating Hippo signaling pathway, the regulatory role of Hippo signaling pathuay on tumor and its effect on radiation effect, aiming to provide new ideas for tumor therapy and the development of radiosensitizing agents.