Abstract: COVID-19 is still the focus of infection prevention and control in the world at present, and has been achieved general basic control under the effective implementation of prevention and control measures in China. However, with the continuous development of the epidemic, there are still small-scale outbreaks. From the perspective of work practice during the COVID-19 epidemic period, the authors preliminarily discussed the management of blood samples from intermediate and high-risk areas in the vitro analysis laboratory of nuclear medicine department in non-designated hospital. The aim of this study is to provide some experience and suggestions for the relevant staff of nuclear medicine laboratories in China, so as to effectively block the transmission route of virus and avoid nosocomial cross infection.