Abstract: At the end of 2019, corona virus disease 2019 (COVID-19) was discovered and spread quickly. It has brought great challenges to the routine work of medical workers. The nuclear medicine department, different from the general imaging department, is mainly responsible for three parts of work including radioactive nuclide diagnosis, nuclide therapy and in vitro analysis. Numerous staff, scattered locations, complex process and inspection items make the management of patients more complicated. Protection work during this period includes not only radioprotection but also strict protection and defense against COVID-19, which called “Dual Defense”. This paper summarizes the special workflow of nuclear medicine department during the outbreak period of COVID-19 based on a series of diagnosis and treatment schemes, protective measures, and guidelines issued by the National Health Commission of the People's Republic of China, as well as actual work experience. The main purpose is to effectively protect people against nuclide radiation and blockade the spread of COVID-19 to the maximum extent simultaneously, reduce the risk of infection for medical staff and improve the medical safety and quality.