Immune-induced aplastic anemia IRM-2 mice model research

Objective To perform traditional blood and immunologic examinations for the pathogenesis of immune-induced aplastic anemia(AA) IRM-2 mice model.

Methods IRM-2 mice were divided into three groups, namely, the control, radiation, and AA model groups. IRM-2 inbred mice in the AA model group were used to set up the AA model by whole body ¹³⁷Cs γ irradiation and eye venous plexus injection with DBA/2 mice thymus cells. After 15 days of radiation, blood and bone marrow cells were counted, and immunologic detection was performed.

Results The peripheral WBC, RBC, bone marrow cells, and reticulocyte(2.38±0.53)×10⁹/L, (8.22±0.21)×10¹²/L, (7.14±1.19)×10⁶/femur, and(50.2±8.9)‰ in the AA model group were lower than those of the control group (8.23±0.41)×10⁹/L, (10.24±0.91)×10¹²/L, (16.5±1.61)×10⁶/femur, and(76.2±9.7)‰. CD4⁺ cells (8.91±2.55)% of the AA model group were lower than those of the control group(16.14±3.09)%, whereas CD8⁺ cells (13.55±2.12)% were higher than those of the control group (6.83±1.14)%. Peripheral blood and immunological indexes of the AA model group were statistically different from those of the control group.

Conclusion IRM-2 mice AA model was successfully established as a good model for the study of AA.