Liang Fu, Shuya He, Baoping Pan, Yang Zeng, Fangzhu Xiao, Shuai Tian, Xianzhou Lu, Bo Huang, Yun Ma. Construction and identification of pprM-complemented Deinococcus radiodurans strain[J]. Int J Radiat Med Nucl Med, 2016, 40(2): 115-119. DOI: 10.3760/cma.j.issn.1673-4114.2016.02.006
Citation: Liang Fu, Shuya He, Baoping Pan, Yang Zeng, Fangzhu Xiao, Shuai Tian, Xianzhou Lu, Bo Huang, Yun Ma. Construction and identification of pprM-complemented Deinococcus radiodurans strain[J]. Int J Radiat Med Nucl Med, 2016, 40(2): 115-119. DOI: 10.3760/cma.j.issn.1673-4114.2016.02.006
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Construction and identification of pprM-complemented Deinococcus radiodurans strain

    Abstract
  • Objective To investigate the function of PprM protein by constructing a pprM-complemented strain of Deinococcus radiodurans.
    Methods HA-pprM gene was amplified by PCR from the plasmid DNA of pGEX-6p-1-pprM(approximately 438 bp) and then inserted into the shuttle expression vector pRADK to construct pRADK-HA-pprM. The correctness of the inserted sequences was confirmed through agarose gel electrophoresis, restriction enzyme digestion, and gene sequencing. The recombinant plasmid was transformed into the pprM mutant strain of Deinococcus radiodurans, and the recombinant protein was analyzed by Western blot.
    Results The recombinant plasmid pRADK-HA-pprM and a complemented strain were obtained. The recombinant protein can be highly expressed in the complemented strain.
    Conclusion This study provided a foundation for further studies on the interaction between the PprM protein and its target DNA.
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