Min Li, Xin He, Zewei Zhou, Qinghui Meng, Saijun Fan. Study on B-cell translocation gene 2 as a novel radiation-induced gene[J]. Int J Radiat Med Nucl Med, 2014, 38(2): 71-74. DOI: 10.3760/cma.j.issn.1673-4114.2014.02.001
Citation: Min Li, Xin He, Zewei Zhou, Qinghui Meng, Saijun Fan. Study on B-cell translocation gene 2 as a novel radiation-induced gene[J]. Int J Radiat Med Nucl Med, 2014, 38(2): 71-74. DOI: 10.3760/cma.j.issn.1673-4114.2014.02.001
shu

Study on B-cell translocation gene 2 as a novel radiation-induced gene

    Abstract
  • Objective To investigate the effects of ionizing radiation on the expression of B-cell translocation gene 2(BTG2) in cancer cells.
    Methods RT-PCR was used to determine the level of BTG2 mRNA, while the expression of BTG2 protein was assayed by Western blot.
    Results Compared with the un-irradiated cells, a single dose of 3 Gy γ-rays caused a significant increase of BTG2 protein in breast cancer cell lines, MCF-7 and MDA-MB-231, and cervical cancer cell lines, C33A and HeLa. Furthermore, irradiation with γ-rays resulted in a dose and time dependent increase of BTG2 at both mRNA and protein levels.
    Conclusion The present study demonstrates, for the first time, that BTG2 is a value response gene to exposure of ionizing radiation, which may provide evidence for BTG2 as a novel marker for radiotherapy of cancer.
    • FullText(HTML)
  • loading
    • References (11)
    • Relative Articles
  • Supplements (0)

Catalog

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return