Objective To improve the reliability of experimental outcome in radio-sensitive drug research by improving the animal model of locally irradiated tumor.
Methods Liver cancer cells H22 were implanted into legs and insteps subcutaneously in mice, respectively, and made them grow into solid tumors. Then the tumors were treated with the γ ray of 137Cs at the dosage of 5 Gy. Measured length, width and height of tumors once per day, and calculated volume of tumors, observed for 24 days.
Results Volume and weight of the irradiated tumor inoculated into legs were no obvious difference(t=0.55、0.70, both P>0.05). But volume and weight of the irradiated tumors inoculated subcutaneously into insteps of the tested mice were obviously lower than that of control mice(t=2.25, P < 0.05 in volume; t=3.14, P < 0.01 in weight).
Conclusion The insteps subcutaneous approach of tumors in mice is a more simple, convenient and practicable operation, and will make the testing result more accurate and reliabe.
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