Li-qing DU, Qiang LIU, Yan WANG, Chang XU, Jia CAO, Yue FU, Feng-hua CHEN, Fei-yue FAN. Regulation of homologous recombination repair protein Rad51 by Ku70[J]. Int J Radiat Med Nucl Med, 2013, 37(5): 272-274. DOI: 10.3760/cma.j.issn.1673-4114.2013.05.005
Citation: Li-qing DU, Qiang LIU, Yan WANG, Chang XU, Jia CAO, Yue FU, Feng-hua CHEN, Fei-yue FAN. Regulation of homologous recombination repair protein Rad51 by Ku70[J]. Int J Radiat Med Nucl Med, 2013, 37(5): 272-274. DOI: 10.3760/cma.j.issn.1673-4114.2013.05.005
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Regulation of homologous recombination repair protein Rad51 by Ku70

    Abstract
  • Objective To explore the regulative effect of non-homologous end joining(NHEJ)protein Ku70 on homologous recombination repair protein Rad51, and to investigate the synergistic mechanism of homologous recombination repair in combination with NHEJ.
    Methods Observed Rad51 protein expression after transfect Ku70 small interfering RNA or Ku70 plasmid DNA into tumor cells using Western blot.
    Results Expression of Rad51 was obviously reduced after pretreated with Ku70 small interfering RNA. And with the increasing expression of Ku70 protein after transfection of Ku70 plasmid DNA PGCsi3.0-hKu70 into tumor cell lines, the Rad51 protein expression was increased.
    Conclusion Ku70 protein has regulating effect on gene expression of Rad51, and it might participate in the collaboration between homologous recombination repair and NHEJ.
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