Qin WANG, Xiao-qiu LIU, Chang XU, Li-qing DU, Zhi-juan SUN, Yan WANG, Qiang LIU, Li SONG, Jin LI, Fei-yue FAN. The function analysis of full-length cDNA sequence from IRM-2 mouse cDNA library[J]. Int J Radiat Med Nucl Med, 2013, 37(5): 262-264. DOI: 10.3760/cma.j.issn.1673-4114.2013.05.002
Citation: Qin WANG, Xiao-qiu LIU, Chang XU, Li-qing DU, Zhi-juan SUN, Yan WANG, Qiang LIU, Li SONG, Jin LI, Fei-yue FAN. The function analysis of full-length cDNA sequence from IRM-2 mouse cDNA library[J]. Int J Radiat Med Nucl Med, 2013, 37(5): 262-264. DOI: 10.3760/cma.j.issn.1673-4114.2013.05.002
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The function analysis of full-length cDNA sequence from IRM-2 mouse cDNA library

    Abstract
  • Objective To identify the function of full-length cDNA sequence from IRM-2 mouse cDNA library.
    Methods Full-length cDNA products were amplified by PCR from IRM-2 mouse cDNA library according to twenty-one pieces of expressed sequence tag. The expression of full-length cDNAs were detected after mouse embryonic fibroblasts were exposed to 6.5 Gy γ-ray radiation. And the effect on the growth of radiosensitivity cells AT5B1VA transfected with full-length cDNAs was investigated.
    Results The expression of No.4, 5 and 2 full-length cDNAs from IRM-2 mouse were higher than that of parental ICR and 615 mouse after mouse embryonic fibroblasts irradiated with γ-ray radiation. And the survival rate of AT5B1VA cells transfected with No.4, 5 and 2 full-length cDNAs was high.
    Conclusion No.4, 5 and 2 full-length cDNAs of IRM-2 mouse are of high radioresistance.
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