Feng-hua CHEN, Xiu SHEN, De-guan LI, Yue FU, Yan-ting GUO, Jin-han WANG, Fei-yue FAN, Qiang LIU. The effects of Tat-SmacN7 protein on radiosensitivity of esophageal carcinoma l09[J]. Int J Radiat Med Nucl Med, 2013, 37(3): 146-149. DOI: 10.3760/cma.j.issn.1673-4114.2013.03.005
Citation: Feng-hua CHEN, Xiu SHEN, De-guan LI, Yue FU, Yan-ting GUO, Jin-han WANG, Fei-yue FAN, Qiang LIU. The effects of Tat-SmacN7 protein on radiosensitivity of esophageal carcinoma l09[J]. Int J Radiat Med Nucl Med, 2013, 37(3): 146-149. DOI: 10.3760/cma.j.issn.1673-4114.2013.03.005
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The effects of Tat-SmacN7 protein on radiosensitivity of esophageal carcinoma l09

    Abstract
  • Objective To investigate the effects of Tat-SmacN7 protein on sensitivity of esophageal carcinoma 109(EC109) to radiation.
    Methods Cells were treated with Tat-SmacN7 peptides, radiation or combination, the inhibition rate of EC109 cells were detected by water-soluble tetrazolium salt-1 assay. The protein level expression of Smac was determined by Western blot.
    Results The cells were resistant to Tat-SmacN7 as a single agent, but it could improve the sensitization of EC109 to radiation. Compared with the Tat-SamcN7 group and the radiation group, the Tat-SmacN7 combined with radiation group was inhibited significantly(24 h:t=16.821 and 9.825, both P < 0.05; 48 h:t=23.553 and 11.930, both P < 0.05). Western blot assay showed that Tat-SmacN7 could increase the expression of Smac protein.
    Conclusions Tat-SmacN7 could improve the radiosensitization of EC109 cell by increasing the expression of Smac.
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