Chang XU, Yan WANG, Li-qing DU, Jia CAO, Qiang LIU. The effects of short hairpin RNA-mediated silencing Net1 on ionizing radiation-induced damage responses[J]. Int J Radiat Med Nucl Med, 2013, 37(3): 135-138. DOI: 10.3760/cma.j.issn.1673-4114.2013.03.002
Citation: Chang XU, Yan WANG, Li-qing DU, Jia CAO, Qiang LIU. The effects of short hairpin RNA-mediated silencing Net1 on ionizing radiation-induced damage responses[J]. Int J Radiat Med Nucl Med, 2013, 37(3): 135-138. DOI: 10.3760/cma.j.issn.1673-4114.2013.03.002
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The effects of short hairpin RNA-mediated silencing Net1 on ionizing radiation-induced damage responses

    Abstract
  • Objective To study the biological roles of the neuroepithelioma transforming gene 1 (Net1)in the cellular responses to ionizing radiation(IR)-induced damage.
    Methods Specific shRNA was used to deplete Net1 in cells. The effects of Net1 depletion on the cellular responses to ionizing radiation were investigated through the colonogenic survival assay and immunoblotting analysis of DNA damage response proteins′ phosphorylation.
    Results Net1-depleted cells were more sensitive to IR, with a significantly increased induction of apoptosis. In response to IR, the phosphorylation levels of ataxia-telangiectasia mutated and checkpoint kinase 2 were much higher in the Net1-depleted cells than that in the control cells.
    Conclusion Net1 protects IR-treated cells from apoptosis and possibly plays an important role in IR-induced damage response and repair.
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