Ying HAN, Bing YANG, Quan TANG, Hui ZHOU, Ti-qiang FAN, Fei-yue FAN, Xiao-dong ZHANG, Yuan-ming SUN. Effects of radiation on macrophage colony stimulating factor in primary osteoblasts[J]. Int J Radiat Med Nucl Med, 2013, 37(1): 5-8. DOI: 10.3760/cma.j.issn.1673-4114.2013.01.002
Citation: Ying HAN, Bing YANG, Quan TANG, Hui ZHOU, Ti-qiang FAN, Fei-yue FAN, Xiao-dong ZHANG, Yuan-ming SUN. Effects of radiation on macrophage colony stimulating factor in primary osteoblasts[J]. Int J Radiat Med Nucl Med, 2013, 37(1): 5-8. DOI: 10.3760/cma.j.issn.1673-4114.2013.01.002
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Effects of radiation on macrophage colony stimulating factor in primary osteoblasts

    Abstract
  • Objective To investigate the radiation effect on the expression of macrophage colony stimulating factor(M-CSF)in primary osteoblasts and the molecular mechanism of bone injury induced by radiation.
    Methods Osteoblasts differentiated from bone marrow stromal cells(BMSCs)were analyzed by real-time PCR and Western blot after 2 Gy or 4 Gy 137Cs γ-irradiation.
    Results M-CSF mRNA and protein expression level were up-regulated after 2 Gy and 4 Gy irradiation(t=-17.329, P < 0.01; t=-3.841, P < 0.05)in osteoblasts. 4 Gy irradiation increased M-CSF mRNA in osteoblast precursors(t=-4.478, P < 0.05), but do not affect the protein expression level.
    Conclusions These results indicated that up-regulated M-CSF in osteoblasts could enhance the function of osteoclasts′ differentiation and maturation which induced by receptor activator of nuclear factor κB ligand after 2 Gy and 4 Gy irradiation. Further more, up-regulated M-CSF expression could enhance osteoclastic bone resorption of mature osteoclasts.
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