The quantitative radiation dose of ³²P by chromosomal aberration

Objective To quantify the radiation dose of ³²P by the G-banding chromosomal aberration rate.

Methods The human blood was irradiated byX-ray of 0.5, 1, 2, 4 Gy respectively. The dose-response curve between the radiation dose and the dicentrics aberration rate was assayed. 74 kBq ³²P colloid was put into culture solution and, after 72 h, the dicentrics aberration rate was observed. The radiation dose of ³²P was assayed by the dose-response curve.

Results The dose-response curve between the radiation dose and the dicentrics aberration rate was y=24.05x-13.34(R²=0.975). The dicentrics aberration rate of 74 kBq ³²P was 18% and the radiation dose of ³²P in 5 ml culture solution during 72 h was 1.3 Gy.

Conclusion The radiation dose of radionuclide can be estimated by chromosomal aberration rate.