Effects of recombinant adenovirus mediated retinoblastoma gene 94 combined with γray on growth of esophageal carcinoma cells

Objective To study the combined effect of exogenous recombinant adenovirus-mediated retinoblastoma gene 94(Ad-Rb94)combined with γ-ray on the growth of esophageal carcinoma cells.

Methods Cell culture were randomly divided into 5 groups: control group, recombinant adenovirus vector containing βgalactosidase gene(Ad-LacZ)group, Ad-Rb94 group, γ-ray radiation group and Ad-Rb94 combined with γray radiation group.EC109 cells were transfected by Ad-Rb94 and exposed to 4 Gy ¹³⁷Cs γ-ray irradiation 6 hours after transfection.The inhibition rate of EC109 cells were detected by MTT assay.

Results EC109 cellstransfected with Ad-Rb94 group, γ-ray radiation group and Ad-Rb94 combined with γ-ray radiation group were all inhibited.The inhibition rate of Ad-Rb94 combined with γ-ray radiation group reached(40.30%±4.2%), significantly higher than Ad-Rb94 group(18.3%±0.4%)and γ-ray radiation group(27.40%±2.9%)(χ²=7.91, χ²= 5.82, P < 0.05).The difference of inhibition rate between γ-ray radiation group and Ad-Rb94 group was extremely significant(χ²=5.12, P < 0.05).

Conclusion The recombinant Ad-Rb94 gene transfection combined with γ-ray shows the synergism forthe inhibition of the growth of EC109 cells.