In vitro study of uptaking ¹⁸F-fluorodeoxyglucose and ¹⁸-fluorodeoxythymidine in human lymphoma Raji cell

Objective To investigate the binding characteristics of ¹⁸F-fluorodeoxyglucose(¹⁸F-FDG) and ¹⁸F-fluorodeoxythymidine(¹⁸F-FLT) with human lymphoma cell Raji and the differences between them.Methods The binding rates of ¹⁸F-FDG and ¹⁸F-FLT was measured under different conditions:1×10⁵~10⁷ cells,1.85~29.6 kBq ¹⁸F-FDG or ¹⁸F-FLT, 0~11.1 mmol/L glucose levels and 20~120 min to measure.Results The binding rate of ¹⁸F-FDG was(50.42±1.07) % at the condition of l×10⁷ cells, 3.7 kBq ¹⁸F-FDG,0~2.78 mmol/L glucose levels, 100 min, while ¹⁸F-FLT was(59.48 ±0.77) % at the same conditions. The difference of binding rates between ¹⁸F-FDG and ¹⁸F-FLT was significant(F=1192.805, P<0.001).Conclusion The uptake of ¹⁸F-FDG and ¹⁸F-FLT in human lymphoma Raji cell is high, and at the same condition, the uptake rate of ¹⁸F-FLT is higher than ¹⁸F-FDG.