Establishment of 25-OH-vitamin D reference interval by radioimmunoassay and its clinical significance

Objective To establish reference interval of 25-OH-vitamin D(25-OH-Vit D)by radioimmunoassay and provide suggestion for clinical applications.Method Collecting 204 healthy persons specimens,and validating and establishing reference interval of 25-OH-Vit D by treatment of outlying observations,iudgement of data distributions and analysis of teat results.Results The reference interval of 25-OH-Vit D established in our laboratory is 16.0~39.7 ng/ml for adolescents and 11.7~40.0 ng/ml for adults.Conclusions The levels of 25-OH-Vit D in humans depend on their age,sex and life style.Some people's vitamin D intake is not enough.Doctors should pay attention to the sufficient 25-OH-Vit D needed when evaluate vitamin D intake.