2007 Vol. 31, No. 5
Display Method:
2007, 31(5): 257-260.
[PDF 340KB]
Abstract:
Objective To evaluate the feasibility and stability of chloramine-T labeling of somatostatin analog Tyr-oetreotide, observe its biodistribution in mice.Methods 131Ⅰ-Tyr-octreotide was prepare for chloramine-T method and the radiochemical purity and colloid content were measured and the biodistribution was studied. 131Ⅰ-Tyr-octreotide was injected via tail vein and measure the radioaction of differents organs at differtent time.Results The best condition of 131Ⅰ-Tyr-octreotide labeling was:(0.2 mol/L) 0.15 ml H3PO4 buffer, Tyr-octreotide (1 g/L) 10 μl, Na131Ⅰ(3.7 MBq)0.1 ml, chloramine-T (30g/L)4μl, Na2S2O5 (30g/L)5μl, reaction time is 2.5 min. The instant radiochemical purity was (97%), and the radiochemical purity of (83%) was remaind after 6h, colloid content is 1.02%. The biodistribution showed high uptake of the tracer in kidney and liver, the stomach intestine was also high, other organs show low uptake.Conclusions Tyr-octreotide was easy to be labeled by chloramine-T. It has achieved high radio-labelling ratio and is a convenient and fast methods. It was quickly cleared from blood and has a high target. 131Ⅰ-Tyr-octreotide may be a promising agent mediated by somatostatin for diagnos and treatment of cancer.
Objective To evaluate the feasibility and stability of chloramine-T labeling of somatostatin analog Tyr-oetreotide, observe its biodistribution in mice.Methods 131Ⅰ-Tyr-octreotide was prepare for chloramine-T method and the radiochemical purity and colloid content were measured and the biodistribution was studied. 131Ⅰ-Tyr-octreotide was injected via tail vein and measure the radioaction of differents organs at differtent time.Results The best condition of 131Ⅰ-Tyr-octreotide labeling was:(0.2 mol/L) 0.15 ml H3PO4 buffer, Tyr-octreotide (1 g/L) 10 μl, Na131Ⅰ(3.7 MBq)0.1 ml, chloramine-T (30g/L)4μl, Na2S2O5 (30g/L)5μl, reaction time is 2.5 min. The instant radiochemical purity was (97%), and the radiochemical purity of (83%) was remaind after 6h, colloid content is 1.02%. The biodistribution showed high uptake of the tracer in kidney and liver, the stomach intestine was also high, other organs show low uptake.Conclusions Tyr-octreotide was easy to be labeled by chloramine-T. It has achieved high radio-labelling ratio and is a convenient and fast methods. It was quickly cleared from blood and has a high target. 131Ⅰ-Tyr-octreotide may be a promising agent mediated by somatostatin for diagnos and treatment of cancer.
2007, 31(5): 261-264.
Abstract:
Cardiovascular molecular imaging is a rapidly evolving discipline and its clinical application is promising. Nuclear medicine is playing a leading role in this field with its special superiority of noninvasive, quantifiability, high sensitivity and specificity. It provides broad opportunities for exploring the pathophysiologic process of cardiovascular diseases and monitoring its gene therapy in the molecular level. In this review, we mainly discuss some basic knowledge on cardiovascular molecular imaging, and then focus on the applied research prospect of nuclear medicine radionuclide imaging.
Cardiovascular molecular imaging is a rapidly evolving discipline and its clinical application is promising. Nuclear medicine is playing a leading role in this field with its special superiority of noninvasive, quantifiability, high sensitivity and specificity. It provides broad opportunities for exploring the pathophysiologic process of cardiovascular diseases and monitoring its gene therapy in the molecular level. In this review, we mainly discuss some basic knowledge on cardiovascular molecular imaging, and then focus on the applied research prospect of nuclear medicine radionuclide imaging.
2007, 31(5): 264-266,273.
Abstract:
Atherosclerosis involves many essential arteries of whole body and it is one of the main diseases that lead adults to death. So it has clinical significance to diagnose atherosclerosis in early stage and to judge its instability. Recent advances in the pathohiology of atherosclerosis have highlighted the inadequacies of the current techniques including the gold standard of X-ray angiography and the need for better imaging approaches. Molecular nuclear medicine can noninvasively detect the number, extent, distribution and component of atherosclerotic plaque, through nuclide imaging with the middle substances during the atherosclerotic process or thrombus on the surface of plaque, and thus can diagnose atherosclerosis in time.
Atherosclerosis involves many essential arteries of whole body and it is one of the main diseases that lead adults to death. So it has clinical significance to diagnose atherosclerosis in early stage and to judge its instability. Recent advances in the pathohiology of atherosclerosis have highlighted the inadequacies of the current techniques including the gold standard of X-ray angiography and the need for better imaging approaches. Molecular nuclear medicine can noninvasively detect the number, extent, distribution and component of atherosclerotic plaque, through nuclide imaging with the middle substances during the atherosclerotic process or thrombus on the surface of plaque, and thus can diagnose atherosclerosis in time.
2007, 31(5): 267-270.
Abstract:
N-acetylglueosaminyl transferase(GnT) is related to the development of tumor and the cancer patients' prognosis by effecting the change of glucose's chain. Study on the transform of glycosyhransferase is benefit to the comprehension of the mechanism of biological behavior. The noninvasive diagnostic and treating methods of tumor will be provided along with the development of new imaging agent of tumor glucose analogue and its mechanism defined clearly.
N-acetylglueosaminyl transferase(GnT) is related to the development of tumor and the cancer patients' prognosis by effecting the change of glucose's chain. Study on the transform of glycosyhransferase is benefit to the comprehension of the mechanism of biological behavior. The noninvasive diagnostic and treating methods of tumor will be provided along with the development of new imaging agent of tumor glucose analogue and its mechanism defined clearly.
2007, 31(5): 270-273.
Abstract:
Human genome project has made a great progress, but the study about the gene expression regulation is still at the beginning. It's found that many diseases such as cancer are related to the deactivation of some gene expressions, whose epigenetic mechanisms involve DNA methylation, histone acetylation and other structural changes in chromatin. That disturbs the normal gene expression regulation and cell multiplication, which finally induce the carcinogenesis. So the re-expression of inactive genes may play a part in inhibiting the carcinogenesis. On the basis of the view, histone deaeetylase (HDAC) inhibitor has a variety of applications in the study of disease and tumor, including the thyroid carcinoma. Furthermore, it comtribute to the mew way of the redifferentication and radioidine therapy against poorly differentiated thyroid carcinoma.
Human genome project has made a great progress, but the study about the gene expression regulation is still at the beginning. It's found that many diseases such as cancer are related to the deactivation of some gene expressions, whose epigenetic mechanisms involve DNA methylation, histone acetylation and other structural changes in chromatin. That disturbs the normal gene expression regulation and cell multiplication, which finally induce the carcinogenesis. So the re-expression of inactive genes may play a part in inhibiting the carcinogenesis. On the basis of the view, histone deaeetylase (HDAC) inhibitor has a variety of applications in the study of disease and tumor, including the thyroid carcinoma. Furthermore, it comtribute to the mew way of the redifferentication and radioidine therapy against poorly differentiated thyroid carcinoma.
2007, 31(5): 274-277.
Abstract:
RGD peptides exist in extracelular matrix of many kinds of organisms,and they can specificly recognise and combin with integrin receptors. After combining with integrin receptors, extrinsic RGD peptides can prevent tumor cells them adhering and migrating, suppress agiogenesis and induce trunor cells apoptosis. And the derivates of RGD peptides can be used as specific imaging libelling and targetal drug dilivery system for tumor therapy.
RGD peptides exist in extracelular matrix of many kinds of organisms,and they can specificly recognise and combin with integrin receptors. After combining with integrin receptors, extrinsic RGD peptides can prevent tumor cells them adhering and migrating, suppress agiogenesis and induce trunor cells apoptosis. And the derivates of RGD peptides can be used as specific imaging libelling and targetal drug dilivery system for tumor therapy.
2007, 31(5): 277-280.
Abstract:
The imaging methods of depressive disorders include structural imaging and functional imaging, the abnormal of cerebral structure in CT and MRI is neither significant nor specific, while the use of PET and SPECT provides depression study in-vivo about regional cerebral blood flow and central neurotransmitter and receptor's structure and function, which further have advanced research on aetiology, pathogenesis and therapy of depressive disorders.
The imaging methods of depressive disorders include structural imaging and functional imaging, the abnormal of cerebral structure in CT and MRI is neither significant nor specific, while the use of PET and SPECT provides depression study in-vivo about regional cerebral blood flow and central neurotransmitter and receptor's structure and function, which further have advanced research on aetiology, pathogenesis and therapy of depressive disorders.
2007, 31(5): 281-284.
Abstract:
Oncology radiotherapy has entered into the biologic eonformal radiotherapy era which is guided by PET and PET-CT. PET and PET-CT can change and improve oncology integration therapy plan and the aim of radiotherapy plan, can make radiotherapy target volume more exact and reduce the observer variation in drawing the target volume, which can reduce target volume bias. More exact target volume leads to higher therapy-effect.
Oncology radiotherapy has entered into the biologic eonformal radiotherapy era which is guided by PET and PET-CT. PET and PET-CT can change and improve oncology integration therapy plan and the aim of radiotherapy plan, can make radiotherapy target volume more exact and reduce the observer variation in drawing the target volume, which can reduce target volume bias. More exact target volume leads to higher therapy-effect.
2007, 31(5): 284-288.
Abstract:
18F-fluorodeoxyglucose (18F-FDG) PET imaging monitoring tumor response in patients undergoing ehemo-and radiotherapy and differentiation residual or recurrent viable tumor and therapy-induced fibrosis or scar tissue has been documented for various solid tumors. Furthermore, there are now several reporis suggesting that quantitative assessment of therapy-induced changes in tumor 18F-FDG uptake may allow prediction of tumor response and patient outcome very early in the course of therapy. In nonresponding patients, trcatnlent may be adjusted according to the individual chemo-and radiosensitivity of the tumor tissue. Early prediction of tumor response to ehenlotherapy and radiotherapy by 18F-FDG PET has enormous potential to "personalize" treatment and to reduce the side-effects and costs of ineffective therapy.
18F-fluorodeoxyglucose (18F-FDG) PET imaging monitoring tumor response in patients undergoing ehemo-and radiotherapy and differentiation residual or recurrent viable tumor and therapy-induced fibrosis or scar tissue has been documented for various solid tumors. Furthermore, there are now several reporis suggesting that quantitative assessment of therapy-induced changes in tumor 18F-FDG uptake may allow prediction of tumor response and patient outcome very early in the course of therapy. In nonresponding patients, trcatnlent may be adjusted according to the individual chemo-and radiosensitivity of the tumor tissue. Early prediction of tumor response to ehenlotherapy and radiotherapy by 18F-FDG PET has enormous potential to "personalize" treatment and to reduce the side-effects and costs of ineffective therapy.
2007, 31(5): 289-291,317.
Abstract:
More and more emphasis has been put on 18F-fluorodeoxyglucose PET, which is regarded as a modem screening tool. We reviewed the clinical application of 18F-fluorodeoxyglucose PET in medical examination as the following aspects:the development of PET imaging, the detection of malignant and benign lesions, false positive and false negative and analyzes of cost-effectiveness. A large amounts of investigations show the significance of the clinical application of 18F-fluorodeoxyglucose PET in medical examination.
More and more emphasis has been put on 18F-fluorodeoxyglucose PET, which is regarded as a modem screening tool. We reviewed the clinical application of 18F-fluorodeoxyglucose PET in medical examination as the following aspects:the development of PET imaging, the detection of malignant and benign lesions, false positive and false negative and analyzes of cost-effectiveness. A large amounts of investigations show the significance of the clinical application of 18F-fluorodeoxyglucose PET in medical examination.
2007, 31(5): 292-295.
Abstract:
The morbidity of lymphomas increased yearly. As a whole-body functional metabolic imaging modality, 18F-FDG PET was very valuable in the diagnosing, staging, selecting treatment program, evaluating therapeutic response and differentiating residual mass. PET had been recognized as a helpful modality because it can provide much more additional information than conventional imaging. However, there was some false positive and false negative.
The morbidity of lymphomas increased yearly. As a whole-body functional metabolic imaging modality, 18F-FDG PET was very valuable in the diagnosing, staging, selecting treatment program, evaluating therapeutic response and differentiating residual mass. PET had been recognized as a helpful modality because it can provide much more additional information than conventional imaging. However, there was some false positive and false negative.
2007, 31(5): 296-299.
Abstract:
Radioadaptive response is a biological defense of which low dose ionizing radiation induces cellular resistance to the genotoxic effects of subsequent challenge irradiation. However, so for molecular mechanism of radioadaptive response remains obscure. Research is mainly involved in activation of the intracellular repair system, cell cycle regulation system, antioxidative stress system and stress-response protein. Signaling factors involved in cell response to radiation include protein kinase C, mitogen-activated protein kinase, p53 tumor suppressor protein, ataxia-telansiectasia mutated, and DNA-dependent protein kinase.
Radioadaptive response is a biological defense of which low dose ionizing radiation induces cellular resistance to the genotoxic effects of subsequent challenge irradiation. However, so for molecular mechanism of radioadaptive response remains obscure. Research is mainly involved in activation of the intracellular repair system, cell cycle regulation system, antioxidative stress system and stress-response protein. Signaling factors involved in cell response to radiation include protein kinase C, mitogen-activated protein kinase, p53 tumor suppressor protein, ataxia-telansiectasia mutated, and DNA-dependent protein kinase.
2007, 31(5): 300-302.
Abstract:
RNA interference (RNAi) is a post-transcriptional gene silencing induced by introduction of double-stranded RNA (dsRNA). The emerging of RNAi provided a new method for glioma gene therapy. During the research, many new potential genes are continually finding out. And the glioma-treating mechanism include inhibiting cell proliferation, inducing cell apoptosis, blocking cell eyele and enhancing cell sensitivity of chemical.
RNA interference (RNAi) is a post-transcriptional gene silencing induced by introduction of double-stranded RNA (dsRNA). The emerging of RNAi provided a new method for glioma gene therapy. During the research, many new potential genes are continually finding out. And the glioma-treating mechanism include inhibiting cell proliferation, inducing cell apoptosis, blocking cell eyele and enhancing cell sensitivity of chemical.
2007, 31(5): 303-306.
Abstract:
An indirect effect induced by ionizing radiation called bystander effect is being highly concentrated. The new achievement by application of advanced tools provides powerful evidence to explain how bystander effects happen and the regulation mechanism.
An indirect effect induced by ionizing radiation called bystander effect is being highly concentrated. The new achievement by application of advanced tools provides powerful evidence to explain how bystander effects happen and the regulation mechanism.
2007, 31(5): 306-308.
Abstract:
The expression of erythropoietin and its receptor is very close related with the hypoxia status in tumor tissues. There are plenty of erythropoietin and erythropoietin receptor in the solid tumors. They are correlative with the resistance to the radiotherapy and chemotherapy. They also promote the invasive and migration. In clinical, there are more than an half of patients with tumor suffering from various extent of anaemia during the process of developing and treating the tumors, It is need to further investigate the use of erythropoietin in tumor patients.
The expression of erythropoietin and its receptor is very close related with the hypoxia status in tumor tissues. There are plenty of erythropoietin and erythropoietin receptor in the solid tumors. They are correlative with the resistance to the radiotherapy and chemotherapy. They also promote the invasive and migration. In clinical, there are more than an half of patients with tumor suffering from various extent of anaemia during the process of developing and treating the tumors, It is need to further investigate the use of erythropoietin in tumor patients.
2007, 31(5): 309-310.
Abstract:
Objective To explore the application value of clinical imageology in the diagnosis of intussusception in infants.Methods Perform uhrasonography and atm-coloclysis X-ray to 50 cases of intussusception in infants.Results The clinical manifestations of infant intussusception are stomachache, crying, vomiting, bloody stools like jam and lump. It showed "cocenric citcle sign" and "bush sign". Atmcoloclysis X-ray examination is useful to its' diagnosis and treatment.Conclusions To acquire significant effect, the doubtful intussusception should perform ultrasonography firstly, and then give atm-coloclysis X-ray examination.
Objective To explore the application value of clinical imageology in the diagnosis of intussusception in infants.Methods Perform uhrasonography and atm-coloclysis X-ray to 50 cases of intussusception in infants.Results The clinical manifestations of infant intussusception are stomachache, crying, vomiting, bloody stools like jam and lump. It showed "cocenric citcle sign" and "bush sign". Atmcoloclysis X-ray examination is useful to its' diagnosis and treatment.Conclusions To acquire significant effect, the doubtful intussusception should perform ultrasonography firstly, and then give atm-coloclysis X-ray examination.
2007, 31(5): 311-314.
Abstract:
Due to its complementary information to standard morphological imaging, flmetional parameters, renal magnetic resonance imaging was developed rapidly these years. The purpose of this article is to review the recent studies of MR technology that renal function related, including traditional imaging sequence analysis, blood oxygenation level dependent imaging, diffusion weighted imaging, arterial spin labeling, contrast enhanced first-pass perfusion, MR renography.
Due to its complementary information to standard morphological imaging, flmetional parameters, renal magnetic resonance imaging was developed rapidly these years. The purpose of this article is to review the recent studies of MR technology that renal function related, including traditional imaging sequence analysis, blood oxygenation level dependent imaging, diffusion weighted imaging, arterial spin labeling, contrast enhanced first-pass perfusion, MR renography.
2007, 31(5): 314-317.
Abstract:
Mesenchymal stein cells (MSCs) are well known for their self-renew and multidifferentiation potentiality. With the transplantation of the MSCs which can promote the regeneration and repair of the injured tissue, a new route for the treatment of dieases is hopeful to be effective. To trace the distribution, migration, proliferation and differentiation of the implanted MSCs, there need effective labeling techniques, especially living labeling techniques.
Mesenchymal stein cells (MSCs) are well known for their self-renew and multidifferentiation potentiality. With the transplantation of the MSCs which can promote the regeneration and repair of the injured tissue, a new route for the treatment of dieases is hopeful to be effective. To trace the distribution, migration, proliferation and differentiation of the implanted MSCs, there need effective labeling techniques, especially living labeling techniques.
2007, 31(5): 318-320.
Abstract:
Dosimetry verification is an important ring in quality assurance for intensity-modulated radiation therapy (IMRT) and is an indispensable procedure in clinlic, with the development of radiation physics technique, new devices and new methods have been applied constantly. They provide us to do the better and more convenience in quality assurance for IMRT, two dimensional ionization chamber array (MatriXX) is a quick dose measurement system, and one of the most advanced two dimensional dose verificatiou system in IMRT at present. It may measure dose distribution and intensity distribution in field size. It may reduce workload and raise efficiency of dosimetry verification in IMRT.
Dosimetry verification is an important ring in quality assurance for intensity-modulated radiation therapy (IMRT) and is an indispensable procedure in clinlic, with the development of radiation physics technique, new devices and new methods have been applied constantly. They provide us to do the better and more convenience in quality assurance for IMRT, two dimensional ionization chamber array (MatriXX) is a quick dose measurement system, and one of the most advanced two dimensional dose verificatiou system in IMRT at present. It may measure dose distribution and intensity distribution in field size. It may reduce workload and raise efficiency of dosimetry verification in IMRT.
Submit
Author Login
Referees
Editor-in-Chief
Editor Center
Email alert
RSS
