Volume 32 Issue 4
Aug.  2008
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Radiosensitivity of filial generation from irradiated human glioma cell line SHG-44

  • Objective To investigate the radiosensitivity and growth features of the progeny of irradiated human glioma cell line SHG-44.Methods The SHG-44 cells were irradiated with 6 MV X ray and the progeny of the cells were cultured.The population doubling time (PDT) was detected pre-and postirradiation. The radiosensitivity of the progeny of irradiated human glioma cell line SHG-44 was measured by clone forming assay.The cell cycle distribution was analysed by flow eytometry.Results The PDT of SHG-44 cells before irradiation Was (22.78±2.61)h.The PDT of irradiated SHG-44 ceils at dose of 10 Gy were (30.46±2.73)h (F=7.878,P<0.05).The surviving fraction of SHG-44 cell was 70.8%,SHG-44-10 cell was 80.6%.SHG-44 cells showed significant G2/M phase decrease and S phase arrest after irradiation. Conclusion Growth delay and declined radiosensitivity are confirmed in the progeny of irradiated SHG-44 cells.
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Radiosensitivity of filial generation from irradiated human glioma cell line SHG-44

    Corresponding author: LI Li, lili371626077@sohu.com
  • 1. Department of Radiation, Suzhou Kowloon Hospital, Shanghai Jiaotong University Medical School Suzhou 215021, China;
  • 2. Department of Radiation, The First Hospital Affiliated to Soochow University, Suzhou 215006, China

Abstract: Objective To investigate the radiosensitivity and growth features of the progeny of irradiated human glioma cell line SHG-44.Methods The SHG-44 cells were irradiated with 6 MV X ray and the progeny of the cells were cultured.The population doubling time (PDT) was detected pre-and postirradiation. The radiosensitivity of the progeny of irradiated human glioma cell line SHG-44 was measured by clone forming assay.The cell cycle distribution was analysed by flow eytometry.Results The PDT of SHG-44 cells before irradiation Was (22.78±2.61)h.The PDT of irradiated SHG-44 ceils at dose of 10 Gy were (30.46±2.73)h (F=7.878,P<0.05).The surviving fraction of SHG-44 cell was 70.8%,SHG-44-10 cell was 80.6%.SHG-44 cells showed significant G2/M phase decrease and S phase arrest after irradiation. Conclusion Growth delay and declined radiosensitivity are confirmed in the progeny of irradiated SHG-44 cells.

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