Volume 25 Issue 3
Jun.  2001
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Citation:

The possibility of dose verification and reconstruction of the early exposure by fluorescence in situ hybridization

  • Received Date: 2001-02-19
  • The paper introduces biological dose reconstruction methodology of the early exposuer and studies on dose response curve in vitro by FISH in recent years and comments the possibility of the technical application to victims'dose verification and reconstruction in abroad. FISH will become a new approach to analyze chromosome aberration and dose reconstruction for early exposure.
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    [2] Xiao Y,Natarajan AT.Non-proportional involvement of Chinese hamster chromosomes 3,4,8 and 9 in X-ray-induced chromosomal aberrations[J].Int J Radiat Biol,1999,75(8):943-951.
    [3] Hsieh W A,Deng W,Chang W P,et al.Alpha coeffi-cient of dose-response for chromosome translocations measured by FISH in human lymphocytes exposed to chronic 60Co gamma rays at body temperature[J].Int J Radiat Biol,1999,75(4):435-439.
    [4] Finno P,Lioyd DC,Edwards AA.Fluorescence in situ hybridization detection of chromosome aberrations in human lymphocytes:applicability to biological des-imetry[J].Int J Radiat Biol,1995,68(4):429-435.
    [5] Lindholm C,Luomahaara S,Koivistoinen A,et al.Comparison of dose-response curves for chromosomal aberrations established by chromosome painting and conventional analysis[J].Int J Radiat Biol,1998,74(1):27-34.
    [6] Barquinero JF,Cigarran S,Caballin M R,et al.Com-parison of X-ray dose-response curves obtained by chromosome painting using conventional and PAINT nomenclatures[J].Int J Radiat Biol,1999,75(12):1557-1566.
    [7] Bauchinger M.Retrospective dose reconstruction of human radiation exposure by FISH/chromosome paint-ing[J].Mutat Res,1998,404(1-2):89-96.
    [8] Bothwell AM,Whitehouse CA,Tawn EJ.The appli-cation of FISH for chrcmosome aberration analysis in relation exposure[J].Radiat Protect Dosi,2000,88(1):7-14.
    [9] Moquet JE,Edwards AA,Lloyd DC,et al.The use of FISH chromosome painting for assessment of old dose of ionizing radiation[J].Radiat Protect Dosim,2000,88(1):27-33.
    [10] Lindholm C,Tekkel M,Veidebaamm T.Persistance of translocations after acidental exposure to ionizing radiation[J].Int J Radiat Biol,1998,74(5):565-571.
    [11] Snigiryova G,Braselmann H,Salassidis D,et al.Ret-rospective bidosimetry of Chernobyl clean-up worker using chromosome painting and conventional chromo-some analysis[J].Int J Radiat Biol,1997,71(2):119-127.
    [12] Stephen A,Pressl S.Chromosome aberrations in hu-man lymphocytes analysed by fluorescence in situ hy-bridization after in vitro irradiation,and in radiation workers,11 years after accidental radiation exposure[J].Int J Radiat Biol,1997,71(3):293-299.
    [13] Lloyd DC,Moquet JE,Oram S,et al.Accidental in-take of tritiated water:a cytogenetic follow-up case on translocation stability and dose reconstruction[J].Int J Radiat Biol,1988,73(5):543-547.
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The possibility of dose verification and reconstruction of the early exposure by fluorescence in situ hybridization

  • Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin, 300192, China

Abstract: The paper introduces biological dose reconstruction methodology of the early exposuer and studies on dose response curve in vitro by FISH in recent years and comments the possibility of the technical application to victims'dose verification and reconstruction in abroad. FISH will become a new approach to analyze chromosome aberration and dose reconstruction for early exposure.

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