Volume 30 Issue 2
Apr.  2006
Article Contents

Citation:

The development and application of time-resolved fluorescence assay

  • Received Date: 2005-06-13
  • Time-resolved fluorescence assay is a new nonisotopic and ultrasensitive detection method of using lanthanide chelate as detectable label and detect lanthanide chelate fluorescence, having a great prospect. In recent years, a serial of assay systems have been developed quickly and have more sensitivity, such as enzyme-amplified time-resolved fluorescence assay, time-resolved quenching assay and molecular beacon. They fit for more different requirement, and have been gotten a broader application in clinical diagnosis and science research.
  • 加载中
  • [1] 赵启仁,时间分辨荧光免疫分析及其应用前景.国外医学·放射医学核医学分册,1992,16(4):182-188.
    [2] 沈建, 林德球, 徐杰.时间分辨荧光免疫分析技术研究现状及进展.生命科学,2004,16(1):55-59.
    [3] Steinkamp T,Karst U.Detection strategies for bioassays based on luminescent lanthanide complexes and signal amplification.Anal Bioanal Chem,2004,380(1):24-30.
    [4] Campbell CN,Gal D,Cristler N,et al.Enzyme-amplified amperometric sandwich test for RNA and DNA.Anal Chem,2002,74(1):158-162.
    [5] Meyer J,Karst U.Enzyme-linked immunosorbent assays based on peroxidase labels and enzyme-amplified lanthanide luminescence detection.Analyst,2001,126(2):175-178.
    [6] Gabourdes M,Bourgine V,Mathis G,et al.A homogeneous time-resolved fluorescence detection of telomerase activity.Anal Biochem,2004,333(1):105-113.
    [7] Steinkamp T,Schweppe F,Krebs B,et al.A tripod ligand as new sensitizer for the enzyme amplified lanthanide luminescence determination of esterase.Analyst,2003,128(1):29-31.
    [8] Ioannou PC,Christopoulos TK.Two-round enzymatic amplification combined with time-resolved fluorometry of Tb3+ chelate for enhanced sensitivity in DNA hybridization assay.Anal Chem,1998,70(4):698-702.
    [9] Karvinen J,Hurskainen P,Gopalakrishnan S,et al.Homogeneous time-resolved fluorescence quenching assay (LANCE) for caspase-3.J Biomol Screen,2002,7(3):223-231.
    [10] Karvinen J,Laitala V,Makinen ML,et al.Fluorescence quenching based assays for hydrolyzing enzymes,application of time resolved fluorometry in assays of caspase,helicase and phosphatase.Anal Chem,2004,76(5):1429-1436.
    [11] Karvinen J,Elomaa A,Makinen ML,et al.Caspase multiplexing:simultaneous homogeneous time-resolved quenching assay (Tru-Point)for caspases 1,3,and 6.Anal Biochem,2004,325(2):317-325.
    [12] Ylikoski A,Elomaa A,Ollikka P,et al.Homogeneous time-resolved fluorescence quenching assay (TruPoint) fornucleic acid detection.Clin Chem,2004,50(10):1943-1947.
    [13] Harma H,Soukka T,Lovgren T,et al.Europium nanoparticles and time-resolved fluorescence for ultrasensitive detection of prostatespecific antigen.Clin Chem,2001,47(3):561-568.
    [14] Kokko L,Sandberg K,Lovgren T,et al.Europium (Ⅲ) chelate-dyed nanoparticles as donors in a homogeneous proximity-based immumoassay for estradiol.Anal Chim Acta,2004,503:155-162.
    [15] Tyagi S,Kramer FR.Molecular beacons:probes that fluoresce upon hybridization.Nat Biotech,1996,14(3):303-308.
    [16] Root DD,Vaccaro C,Zhang Z,et al.Detection of single nucleotide variations by a hybridization proximity assay based on molecular beacons and luminescence resonance energy transfer.Biopolymers,2004,75(1):60-70.
    [17] 欧阳松应,杨冬, 欧阳红生,等.实时荧光定量PCR技术及其应用.生命的化学,2004,24(1):74-76.
    [18] Nurmi J,Wikman T,Karp M,et al.High-performance real-time quantitative RT-PCR using lanthanide probes and a dual-temperature hybridization assay.Anal Chem,2002,74(14):3525-3532.
    [19] Rudi K,Moen B,Dromtorp SM,et al.Use of ethidium monoazide and PCR in combination for quantification of viable and dead cells in complex samples.Appl Environ Microbiol,2005,71(2):1018-1024.
    [20] Wu M,Lakowicz JR,Geddes CD.Enhanced lanthanide luminescence using silver nanostructures:opportunities for a new class of probes with exceptional spectral characteristics.J Fluoresc,2005,15(1):53-59.
    [21] Kemper C,Berggren K,Diwu Z,et al.An improved,luminescent europium-base stain for detection of electroblotted proteins on nitrocellulose or polyvinylidene difluoride membranes.Electrophoresis,2001,22(5):881-889.
    [22] Albrecht H,Zbinden P,Rizzi A,et al.High throughput screening of beta-amyloid secretion inhibitors using homogeneous time-resolved fluorescence.Comb Chem High Throughput Screen,2004,7(8):745-756.
    [23] Claret EJ,Ouled-Diaf J,Seguin P.Homogeneous time resolved fluorescence assay to measure histamine release.Comb Chem High Throughput Screen,2003,6(8):789-794.
    [24] Enomoto K,Araki A,Nakajima T,et al.High-throughput miniaturized immunoassay for human interleukin-13 secreted from NK3.3 cells using homogeneous time-resolved fluorescence.J Pharm Biomed Anal,2002,28(1):73-79.
    [25] Ferrer M,Zuck P,Kolodin G,et al.Miniaturizable homogeneous time-resolved fluorescence assay for carboxypeptidase B activity.Anal Biochem,2003,317(1):94-98.
    [26] Nurmi J,Kiviniemi M,Kujanpaa M,et al.High-throughput genetic analysis using time-resolved fluorometry and closed-tube detection.Anal Biochem,2001,299(2):211-217.
    [27] Moore K J,Turconi S,Miles-williams A,et al.A homogeneous 384-well high throughput screen for novel tumor necrosis factor receptor:ligand interactions using time resolved energy transfer.J Biomol Screen,1999,4(4):205-214.
    [28] Maurel D,Kniazeff J,Mathis G,et al.Cell surface detection of membrane protein interaction with homogeneous time-resolved fluorescence resonance energy transfer technology.Anal Biochem,2004,329(2):253-262.
    [29] Zhou G,Cummings R,Hermes J,et al.Use of homogeneous time-resolved fluorescence energy transfer in the measurement of nuclear receptor activation.Methods,2001,25(1):54-61.
  • 加载中
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Article Metrics

Article views(1134) PDF downloads(4) Cited by()

Related
Proportional views

The development and application of time-resolved fluorescence assay

  • Department of Experimental Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China

Abstract: Time-resolved fluorescence assay is a new nonisotopic and ultrasensitive detection method of using lanthanide chelate as detectable label and detect lanthanide chelate fluorescence, having a great prospect. In recent years, a serial of assay systems have been developed quickly and have more sensitivity, such as enzyme-amplified time-resolved fluorescence assay, time-resolved quenching assay and molecular beacon. They fit for more different requirement, and have been gotten a broader application in clinical diagnosis and science research.

    HTML

Reference (29)

Catalog

/

DownLoad:  Full-Size Img  PowerPoint
Return
Return