Volume 33 Issue 4
Aug.  2009
Article Contents

Citation:

Progress in measurement of free radicals in biological systems

  • Received Date: 2009-02-22
  • Free radicals generation and oxidative stress are involved in many pathological processes including neuro-degenerated diseases, diabetes, tumorigenesis and radiation damage. Measurement of free radicals is of importance in the field of radiation protection and radiation damage. The electron spinning resonance (ESR) techniques for measurement of free radicals in biological systems are summarized according to the literature and the author's research work in this review. The recent emerging imrauno-spin trapping method for analysis of protein radicals and D N A radicals is also introduced.
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  • [1] Mosley RL,Benner EJ,Kadiu I,et al.Neuroinflammation,oxidative stress and the pathogenesis of Parkinson's disease.Clin Neupochcm Res,2006,6(5):261-281.
    [2] Block ML NADPH oxidase as a therapeutic target in Alzheimer's disease.BMC Neurosci,2008,9(Suppl 2):S8.
    [3] Niebrój-Dobosz I,Dziewulska D,Kwiecifiski H.Oxidative damage to proteins in the spinal cord in amyotrophic lateral sclerosis(ALS).Folia Neuropathol,2004,42(3):151-156.
    [4] Liu J,Qian SY,Guo Q,et al.Cadmium generates reactive oxygen-and carbon-centered radical species in rats:Insights from in vivo spin-trapping studies.Free Radic Biol Med,2008,45(14):475-481.
    [5] Pan CJ,Schmitz DA,Cho AK,et al.Inherent redox properties of diesel exhaust particles:catalysis of the generation of reactive oxygen species by biological reductants.Toxicol Sci,2004,81(1):225-232.
    [6] National Research Council Authoring Organizations.Health risks from exposure to low levels of ionizing irradiation:BEIR Ⅶ phase 2.National Research Council of the National Academies.Washington:The National Academies Press,2006:43-90.
    [7] Nakamura N,Miyazawa C,Sawada S et al.A elose correlation between electron spin resonance (ESR) dosimetry form tooth enamel and cytogenetic dosimetry from lymphocytes of Hiroshima atomic-bomb survivors.Int J Radiat Biol,1998,73(6):619-627.
    [8] Miura Y,Anzai K,Ueda JI,et al.Novel approach to in vivo screening for radioprotective activity in whole mice:In vivo electron spin resonance study probing the redox reaction of nitrioxyl.J Radiat Res(Tokyo),2000,41(2):103-111.
    [9] Ward JF.The complexity of DNA damage:relevancc to biological consequences.Int J Radiat Biol,1994,66(5):427-432.
    [10] Borisenko GG,Martin I,Zhao Q,et al.Nitroxide scavenge myeloperoxidase-catalyzed thiyl radicals in model systems and in cells.J Am Chem Soc,2004,126(30):9221-9232.
    [11] Ranguelova K,Mason RP.New insights into the detection of sulfur trioxide anion radical by spin trapping:radical trapping versus nucleophilic addition.Free Radic Biol Med,2009,47(2):128-134.
    [12] Kim SR,Jockusch S,Itagaki Y,et al.Mechanisms involved in A2E oxidation.Exp Eye Res,2008,86(6):975-982.
    [13] Timoshin AA,Drobotova DY,Lakomkin VL,et al.Estimation of nitric oxide level in vivo by microdialysis with water-soluble iron-N-methyl-D-dithiocarbamate complexes as NO traps:a novel approach to nitric oxide spin trapping in animal tissues.Nitric Oxide,2008,19(4):338-344.
    [14] Diepart C,Jordan BF,Gallez B,A New EPR oximetry protocol to estimate the tissue oxygen consumption in vivo.Radiat Res,2009,172(2):220-225.
    [15] Takeshita K,Chi C,Hirata H,et al.In vivo generation of free radicals in the skin of live mice under ultraviolet light,measured by L-band EPR spectroscopy.Free Radic Biol Med,2006,40(5):876-885.
    [16] Bacic G,Nilges MJ,Magin RL,et al.In vivo localized ESR spectroscopy reflecting metabolism.Magn Reson Med,1989,10(2):266-272.
    [17] Zhelev Z,Bakalova R,Aoki I,et al.Nitroxyl radicals for labeling of conventional therapeutics nwiinvasive magnetic resonance imaging of their permeability for blood-brain banicr relationship between structure,blood clearance,and MRI signal dynamic in the brain.Mol Pharrn,2009,6(2):504-512.
    [18] Matsumoto K,Uteumi H.Development of separable electron spin resonance-computed tomography imaging for multiple radical species:an application to.OH and.NO.Biophys J,2000,79(6):3341-3349.
    [19] Ramirez DC,Goroez-Mejiba SE,Mason RP.Immuno-spin trapping analyses of DNA radicals.Nat Protoc,2007,2(3):512-522.
    [20] Mason RP.Using anti-5,5-dimethyl-1-pyrroline N-oxide (anti-DMPO) to detect protein radicals in time and space with immuno-spin trapping.Free Radic Biol Med,2004,36(10):1214-1223.
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Progress in measurement of free radicals in biological systems

  • Department of Radiation Medicine and Environmental Meaicme, China Institute for Radiation Protection, Taiyuan 030006, China

Abstract: Free radicals generation and oxidative stress are involved in many pathological processes including neuro-degenerated diseases, diabetes, tumorigenesis and radiation damage. Measurement of free radicals is of importance in the field of radiation protection and radiation damage. The electron spinning resonance (ESR) techniques for measurement of free radicals in biological systems are summarized according to the literature and the author's research work in this review. The recent emerging imrauno-spin trapping method for analysis of protein radicals and D N A radicals is also introduced.

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