用<sup>99m</sup>Tc标记胰岛素样生长因子1类似物的方法学研究

范光磊; 吴翼伟; 章斌

用^99mTc标记胰岛素样生长因子1类似物的方法学研究

范光磊 , 吴翼伟 , 章斌

文章导航 > 国际放射医学核医学杂志 > 2007, 31 > 4: (208-211)

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用^99mTc标记胰岛素样生长因子1类似物的方法学研究

215006 苏州, 苏州大学附属第一医院核医学科
基金项目:
江苏省苏州市社会发展基金项目(SSY0617)
中图分类号: R817.4

Investigations of labeling insulin-like growth factor 1 analogue with ^99mTc

Department of Nuclear Medicine, The First Affiliated Hospital to Smkou Unwersity, Jiangsu Suzhou 215006, China
CLC number: R817.4

摘要: 目的建立^99mTc标记胰岛素样生长因子1类似物(IGF-1A)的方法,探讨其标记条件。方法通过预锡化法标记IGF-1A,改变标记条件:Tween 80的用量从0~10μl,在0.25~6 h不同时间点测定标记率,SnCl₂·2H₂O质量浓度0.75~4 g/L,IGF-1A的用量20~100μg,淋洗液的体积10~200μl,加入生理盐水或人血清后1 h~24 h测定放化纯度,纸层析法测定标记率及胶体水平。结果 ^99mTc-IGF-1A最高标记率为(94.43±0.75)%,放射性胶体质量分数为(3.47±0.71)%。室温下放置6h标记率为(85.57±2.81)%,加入人血清后放置24h标记率为(54.07±3.86)%。结论上述预锡化法进行^99mTc标记IGF-1A的最佳标记方法为:浓盐酸溶解SnCl₂·2H₂O后用葡萄糖酸钠溶液配成3g/L,吸取100μl后加入40μl IGF-1A(2g/L);300μl Na₃PO₄和2μl 0.1%Tween 80混匀后加入50μl ^99mTcO₄-新鲜淋洗液;在IGF-1A体系中加入淋洗液体系,混匀,室温下反应0.5h;加入500μl NaH₂PO₄-将pH值调节到7左右。该方法简捷且具有较高标记率和良好稳定性。
- 胰岛素样生长因子1 /
- 锝 /
- 同位素标记
Abstract: Objective To establish a useful and stable method for labeling insulin-like growth factor I analogue(IGF-1A) with ^99mTc. Methods The "pretinning" method was adopted to label IGF-1A. Several labeling conditions were tested. The volume of Tween 80 was from 0 to 10μl, the labeling efficiency was determined from 0.25 h to 6 h after labeling, SnCl₂·2H₂0 from 0.75g/L to 4g/L, the amount of IGF-1A from 20 to 100μg, ihe volume of ^99mTc perrhenate was from 10 to 200μL The in vitro stability of ^99mTc-IGF-IA was analyzed by using Iranian serum or sodium chloride as challenging agent, and the labeling efficiency was determined from 1 h to 24 h after added challenging agent. Results The labeling efficiency of ^99mTc-IGF-1A could reach(94.43±0.75)% and the mass fraction of radiocolloid was(3.47±0.71)%. It was(85.57±2.81)% after incubation 6h at room temperature, and was(54.07±3.86)% after incubation 24 h with human serum. Conclusions The optimum labeling method was 100μl stannous chloride(3g/L) dissolved in sodium gluconate, 40μl ICF-1A(2g/L), 300μl Na₃PO₄, 2μl 0.1% Tween 80, 50μl ^99mTc perrhenate, incubation 0.5h at room temperature, then added 500μl NaH₂PO₄. This method of labeling IGF-1A with ^99mTc using SnCl₂·2H₂O and sodium gluconate was stable and high labeling efficiency was obtained.
- Insulin-like growth factor-1 /
- Technetium /
- Isotope labeling
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用^99mTc标记胰岛素样生长因子1类似物的方法学研究

215006 苏州, 苏州大学附属第一医院核医学科

收稿日期: 2006-12-23

基金项目: 江苏省苏州市社会发展基金项目(SSY0617)

关键词:

摘要: 目的建立^99mTc标记胰岛素样生长因子1类似物(IGF-1A)的方法,探讨其标记条件。方法通过预锡化法标记IGF-1A,改变标记条件:Tween 80的用量从0~10μl,在0.25~6 h不同时间点测定标记率,SnCl₂·2H₂O质量浓度0.75~4 g/L,IGF-1A的用量20~100μg,淋洗液的体积10~200μl,加入生理盐水或人血清后1 h~24 h测定放化纯度,纸层析法测定标记率及胶体水平。结果 ^99mTc-IGF-1A最高标记率为(94.43±0.75)%,放射性胶体质量分数为(3.47±0.71)%。室温下放置6h标记率为(85.57±2.81)%,加入人血清后放置24h标记率为(54.07±3.86)%。结论上述预锡化法进行^99mTc标记IGF-1A的最佳标记方法为:浓盐酸溶解SnCl₂·2H₂O后用葡萄糖酸钠溶液配成3g/L,吸取100μl后加入40μl IGF-1A(2g/L);300μl Na₃PO₄和2μl 0.1%Tween 80混匀后加入50μl ^99mTcO₄-新鲜淋洗液;在IGF-1A体系中加入淋洗液体系,混匀,室温下反应0.5h;加入500μl NaH₂PO₄-将pH值调节到7左右。该方法简捷且具有较高标记率和良好稳定性。