摘要:
目的 探讨抗人肝癌188Re-免疫(Hepama-1)磁性纳米微粒免疫学活性、体内生物学分布、肝靶向性及抑瘤作用。方法 对比188ReO4-、188Re-Hepama-1、188Re-磁性纳米微粒,研究尾静脉注射188Re-免疫磁性纳米微粒后4h、24h在昆明小鼠体内的分布情况;在肝区外加磁场及无磁场状态下,研究新西兰大白兔体内的肝磁靶向性;采用溴化四甲基唑法,研究4种188Re标记物对肝癌细胞株SMMC-7721的抑制效果。结果 188Re-免疫磁性纳米微粒在肝内摄取量最大;在磁区肝组织放射活性较非磁区肝组织明显增加,二者的放射活性比值为1.87;对肝癌细胞株SMMC-7721半数抑制放射性活度仅为188ReO4-的1/4左右。结论 188Re-磁性纳米微粒具有良好的磁感应性能、免疫活性圾明显的肝靶向性。
Abstract:
Objective To investigate a new biologic-superparamagnetic nanoparticles's characteristics of immunological activity, biological distributing in vivo, targeting and inhibiting tumoreffect. Methods The experimental group 188Re-Hepama-1-superparamagnetic nanoparticles, and control groups, including 188ReO4-, 188Re-Hepama-1, and 188Re-superparamagnetic nanoparticles, were set up. The distributions were measured after injection 4 h and 24 h by caudal vein of Kuming mice. The magnetic targeting experiments in vivo were done with and without magnetic field in liver after injection in New Zealand rabbit. The inhibiting tumor effect on hepatic cancer cell lines SMMC-7721 of the above four 188Re labeled products were measured by mono nuclear cell direct cytotoxicity assay method. Results After injection 4 h and 24 h by vein, the liver taking was highest in group 188Re-Hepama-1-superparamagnetic nanoparticles. The radiative activity in liver in magnetism zoo was higher than in non magnetism zoo in 188Re-Hepama-1-superparamagnetic nanoparticles after applying magnetic field in left lobe of liver, and the ratio of in magnetism zoo to non magnetism zoo was 1.87. And the half effective inhibition radioactive concentrations (IC50) in 188Re-Hepama-1-superparamagnetic nanoparticles was one forth of 188ReO4-. Conclusion 188Re-Hepama-1-superparamagnetic nanoparticles showed its fine stability in intro, good immunological activity and significant liver target.
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