摘要:
目的 研究188Re标记胰岛素样生长因子l类似物(IGF-1A)在荷人胰腺癌裸鼠体内的分布及其显像。方法 ①直接法标记188Re-IGF-1A并测定标记率。②建立荷人胰腺癌Patu8988裸鼠模型。③188Re-IGF-1A经瘤内注射荷人胰腺癌裸鼠瘤内,分别于注射后15 min、1 h、4 h、24 h、3 d、5 d进行SPECT平面显像。④188ReO4-经瘤内注射后15 min、1 h、2 h、4 h、24 h进行显像,取各时间组裸鼠(n=4)脏器和肿瘤组织,计算每克组织百分注入剂量(%ID/g)及肿瘤/非肿瘤组织放射性摄取比值(T/NT)。结果 ①188Re-IGF-1A标记率为(94.07±0.32)%。②瘤内注射188Re-IGF-1A后,肿瘤部位放射性积聚量4 h内差异无统计学意义(F=1.622,P>0.05),且随时间延长,肿瘤与其他脏器的T/NT呈上升趋势,其中肿瘤/肌肉在5 d时最高,达到6531.79±4930.26.③瘤内注射188ReO4-后,在体内初始主要分布于甲状腺、胃、肿瘤、血液,随时间延长,肿瘤部位放射性计数迅速下降。④在24 h,瘤内注射188Re-IGF-1A组肿瘤及肾脏内% ID/g较188ReO4-组高,两者有统计学差异(t=5.877,t=13.287,P<0.01);两组肿瘤内% ID/g比值在24 h达到最高,为74.10倍。⑤瘤内注射188Re-IGF-1A后,SPECT平面显像见瘤内浓聚,5 d时仅见肿瘤部位显影。结论 188Re-IGF-1A对胰腺癌具有良好的亲和力,在肿瘤部位有较高的T/NT,可望作为胰腺癌治疗的药物。
Abstract:
Objective To evaluate the biodistribution and planar gamma carnera jmaging characteristics of 188Re labeled insulin-like growth factor 1 analogue(188Re-IGF-1A)in tumor-bearing mice.Methods ①To label IGF-1A with 188Re directly and to determine the labeling efficiency.②To establish nude mice model which beating human pancreatic carcinoma cell Patu8988.③To scan those nude mice at 15 min,1 h,4 h,24 h,3 d and 5 d after intratumor injection with 188Re-IGF-1A into their tumors.④To scan those nude mice at 15min,1 h,2h,4 h and24 h after intratumor injection with 188ReO4-into their tumors.To calculate the tumor to normal tissue ratio(T/NT)and the percentages of injected dose per gram tissue(%ID/g)of different organs.Results ①The labeling efficiency of 188Re-IGF-1A was(94.07±0.32)%.②The largest uptake of tumors was(42.38+17.82)%ID/g at 4 h after injection of 188Re-IGF-1A.Then the tumor to normal tissue ratios increased and the largest Rnnor to mugcle ratio was 6531.79±4930.26 at 5 d after injection.③188ReO4- wag major distributed in thyroid glands,stomachs,tumors and blood in nude mice after injection at first.Then%ID/g decreased rapidly in tumors.④The difference of%ID/g wag siguificant(t=5.877,t=13.287,P<0.01) in tumors and kidneys between 188Re-IGF-1A group than in 188ReO4- group.The largest ratio of tumors in the two groups was 74.10 at 24 h after injection.⑤After being injected,188Re-IGF-1A formed clear images in tumom.5 d later,nothing but tumors can be seen.Conclusions 188Re-IGF-1A has good affinity with human pancreatic cancer,and the tumor to muscle ratios in nude mice is high.So 188Re-IGF-1A is expected to be used for targeting therapy of human pancreatic carcinoma.
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