时间分辨荧光分析技术的研究进展及应用

周伟玲; 赵启仁

时间分辨荧光分析技术的研究进展及应用

周伟玲 , 赵启仁

文章导航 > 国际放射医学核医学杂志 > 2006, 30 > 2: (103-106)

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时间分辨荧光分析技术的研究进展及应用

300192 天津, 中国医学科学院、中国协和医科大学放射医学研究所实验核医学室
基金项目:
天津市自然科学基金资助项目(043610211)
中图分类号: R446.62

The development and application of time-resolved fluorescence assay

Department of Experimental Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China
CLC number: R446.62

摘要: 时间分辨荧光分析(TRFA)技术是一种以镧系元素螫合物为标记物,测量其发射荧光的超灵敏的无放射性污染的标记分析技术,在生物分析中得到了广泛的应用。在近些年,研究发展了酶放大时间分辨荧光分析、时间分辨猝灭分析和分子信标等分析系统,其灵敏度更高,满足了不同的需求,使TRFA技术在临床诊断和科学研究中得到了更广泛的应用。
- 荧光免疫测定 /
- 稀土元素 /
- 荧光共振能量转移
Abstract: Time-resolved fluorescence assay is a new nonisotopic and ultrasensitive detection method of using lanthanide chelate as detectable label and detect lanthanide chelate fluorescence, having a great prospect. In recent years, a serial of assay systems have been developed quickly and have more sensitivity, such as enzyme-amplified time-resolved fluorescence assay, time-resolved quenching assay and molecular beacon. They fit for more different requirement, and have been gotten a broader application in clinical diagnosis and science research.
- Fluoroimmuno assay /
- Lanthanide series elements /
- Fluorescence resonance energy transfer
本作品遵循Signature-Non-Commercial Use 4.0 International (CC BY-NC 4.0)协议

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时间分辨荧光分析技术的研究进展及应用

300192 天津, 中国医学科学院、中国协和医科大学放射医学研究所实验核医学室

收稿日期: 2005-06-13

基金项目: 天津市自然科学基金资助项目(043610211)

关键词:

摘要: 时间分辨荧光分析(TRFA)技术是一种以镧系元素螫合物为标记物,测量其发射荧光的超灵敏的无放射性污染的标记分析技术,在生物分析中得到了广泛的应用。在近些年,研究发展了酶放大时间分辨荧光分析、时间分辨猝灭分析和分子信标等分析系统,其灵敏度更高,满足了不同的需求,使TRFA技术在临床诊断和科学研究中得到了更广泛的应用。