mRNA差异显示技术及其在辐射研究中的应用

朱俊东

朱俊东

文章导航 > 国际放射医学核医学杂志 > 2000, 24 > 1: (31-35)

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mRNA差异显示技术及其在辐射研究中的应用

朱俊东

400038 重庆, 第三军医大学防原医学教研室

作者简介: 朱俊东(1968-),男,湖北黄冈人,第三军医大学营养与食品卫生学教研室讲师,在读博士生,主要从事放射损伤研究。;

中图分类号: Q503;Q274

mRNA Differential Display and its Application in Radiation Research

ZHU Jun-dong

Department of Radiation medicine, Third Military Medical University, Chongqing 400038, China
CLC number: Q503;Q274

摘要: mRNA差异显示技术是建立在AP-PCR(锚定引物聚合酶链式反应)和RT-PCR(逆转录聚合酶链式反应)技术基础上的一种筛选别差异表达基因的有效方法。该自建立以来,一直在不断地完善少发展,已成为一项重要的分子生物学技术手段。目前,mRNA差异显示技术已在辐射研究领域得到初步应用,但还未充分发挥其巨大的潜能。应用该技术研究辐射损伤和损伤后修复的相关基因,有利于阐明辐射损伤和损伤后修复病理过程的分子机制,从而对辐射防治实践具有重要指导意义。
- mRNA差异显示技术 /
- 辐射 /
- 差异表达基因
Abstract: mRNA differential display, based upon the techmiques of anchored primer-polymerase chain reaction and reverse transcription-pohymerase chain reaction,is an effective method for the detection of differentially expressed genes. Since its initial description, it has been an important molecular biology technique with continuous improvements and developments. The application of this technique for the identification of radiation injuries and repair related genes will help to understand the molecular mechanisms of these pathological processes,as well as provide guidance for prevention and therapy of radiation injuries.
- mRNA differential display /
- radiation /
- differentially expressed gene
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mRNA差异显示技术及其在辐射研究中的应用

朱俊东

作者简介:朱俊东(1968-),男,湖北黄冈人,第三军医大学营养与食品卫生学教研室讲师,在读博士生,主要从事放射损伤研究。

400038 重庆, 第三军医大学防原医学教研室

收稿日期: 1999-10-05

关键词:

摘要: mRNA差异显示技术是建立在AP-PCR(锚定引物聚合酶链式反应)和RT-PCR(逆转录聚合酶链式反应)技术基础上的一种筛选别差异表达基因的有效方法。该自建立以来,一直在不断地完善少发展,已成为一项重要的分子生物学技术手段。目前,mRNA差异显示技术已在辐射研究领域得到初步应用,但还未充分发挥其巨大的潜能。应用该技术研究辐射损伤和损伤后修复的相关基因,有利于阐明辐射损伤和损伤后修复病理过程的分子机制,从而对辐射防治实践具有重要指导意义。