不同剂量<sup>60</sup>Coγ射线照射正常人淋巴母细胞后的差异表达基因分析

肖瑶; 杨剑; 高娴; 秦阳华; 孙顶; 韩玲

不同剂量⁶⁰Coγ射线照射正常人淋巴母细胞后的差异表达基因分析

肖瑶 , 杨剑 , 高娴 , 秦阳华 , 孙顶 , 韩玲

文章导航 > 国际放射医学核医学杂志 > 2008, 32 > 2: (101-105)

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不同剂量⁶⁰Coγ射线照射正常人淋巴母细胞后的差异表达基因分析

200433 上海, 第二军医大学海军医学系放射医学教研究
基金项目:
国家自然科学基金资助项目(10575130)

Different gene expression of normal lymphoblastoid cells which exposure to different dose of ⁶⁰Coγ-ray

Department of Radiation Medicine in Navy Medicine Faculty, the Second Military Medical University, Shanghai 200433, China

摘要: 目的研究不同剂量γ射线照射正常人淋巴母细胞AHH-1的基因差异表达,探讨生物学效应的差异。方法 ⁶⁰Coγ射线照射AHH-1,用人cDNA芯片检测照射后8h AHH-1细胞和正常细胞mRNA表达,将芯片分析数据进行聚类分析、判别比较,筛选差异表达基因。结果数据分析筛选后,仅与2.0 Gy照射关系密切的差异表达基因23个;仅在0.5 Gy照射中变化的基因5个;2.0Gy与0.5Gy两组比较变化一致的基因有5个。结论不同剂量γ射线照射AHH-1诱导明显的基因差异表达,其中部分基因表达的改变可能是辐射生物效应发生的关键因素。
- 剂量效应关系,辐射 /
- 基因表达谱 /
- 淋巴母细胞
Abstract: Objective To study on the gene expression of normal lymphoblastoid cells(AHH-l) which exposure to difference dose of ⁶⁰Coγ-ray, analyses the essential different biological effect. Methods Human AHH-I normal line was irradiated by ⁶⁰Coγ-rays. Used human cDNA microarray to develop the transcriptional levels of the genes by hybridizing the mRNA of cells 8 h after exposured in different dose and the control cells. Cluster analysis, discrimination and bolting were used to filter the effective genes of differential expression. Results The results of data analysis showed 23 genes of differential expression closely related to biological effect of 2.0 Gy radiation, 5 genes express changed only by 0.5 Gy radiation, 5 genes express apparently both in 2.0 Gy and 0.5 Gy radiation. Conclusion The different dose γ-rays radiation-induced significant changes in gene expression, such as PAPLN, TP53INPI, PTENP1, FOS and TPR seem to be some important components of cellular radioresponse.
- Dose-response relationship, radiation /
- Gene expression profiling /
- Lymphoblastoid eells
本作品遵循Signature-Non-Commercial Use 4.0 International (CC BY-NC 4.0)协议

[1]	Yang K, Cai Z, Li J, et al. A stable gene selection in microarray data analysis[J]. BMC Bioinfomatics, 2006, 7:228.
[2]	Albanesea J, Martensa K, Karkanitesa LV, et al. Multivariate analysis of low-dose radiation-assoclated changes in cytokine gene expression profiles using microarray technology[J]. Exp Hematolo, 2007, 35(4Suppl1):47-54.
[3]	Chaudhry MA. Bystander effect:Biologlcal endpoints and microarray analysis[J]. Mutat Res, 2006, 597(1-2):98-112.
[4]	Lindsay KJ, Coates PJ, Lorimore SA, et al. The genetic basis of tissue responses to ionizing radiation[J]. Br J Radiol, 2007, 80(Spec 1):S2-S6.
[5]	Kramerova IA, Kawaguchi N, Fessler LI, et al. Papilin in development:a pericellular protein with a homology to the ADAMTS metalloproteinases[J]. Development, 2000, 127(24):5475-5485.
[6]	Okamura S, Arakawa H, Tanaka T, et al. p53-inducible gene, regulates p53-dependent apoptosis[J]. Mol Cell, 2001, 8(1):85-94.
[7]	Tomasini R, Samir AA, Carrier A, et al. TP53INPIs and homeodomain-interaeting protein kinase-2(HIPK2) are partners in regulating p53 activity[J]. J Biol Chem, 2003, 278(39):37722-37729.
[8]	Dahia PL, FitzGerald MG, Zhang X, et al. A highly conserved processed PTEN pseudogene is located on chromosome band 9p21[J]. Oncogene, 1998, 16(18):2403-2406.
[9]	Greeo A, Pierotti MA, Bongarzone I, at el. TRK-TI is a novel oncogene formed by the fusion of TPR and TRK genes in human papillary, thyroid carcinomas[J]. Oncogene. 1992, 7(2):237-242.
[10]	Hase ME, Cordes VC. Direct interaction with nup153 mediates binding of Tpr to the periphery of the nuclear pore complex[J]. Mol Biol Cell, 2003, 14(5):1923-1940.
[11]	Krull S, Thyberg J, BjiSrkroth B, at el. Nucleoporing as components of the nllclear pore complex core structure and Tpr as the architectural element of the nuclear basket[J]. Mol Biol Cells.2004, 15(9):4261-4277.
[12]	Tratner I, Ofir R, Verma IM. Alteration of a cyclic AMP-dependent protein kinase phosphorylation site in the c-FOS protein augments its transforming potential[J]. Mol Cell Biol. 1992, 12(3):998-1006.
[13]	Liu G, Ding W, Liu X, et al. c-Fos is required for TGF-betal production and the associated paracrine migratory effects of human colon carcinoma cells[J]. Mol Carcinog, 2006, 45(8):582-593.
[14]	Zhang J, Zhang L, Jiao H, et al. c-FOS facilitates the acquisition and extinction of cocaine-induced persistent changes[J]. J Neurosci, 2006, 26(51):13287-13296.
[15]	Sasaki T, Kojima H, Kishimoto R, et al. Spatiotemporal regulation of c-FOS by ERK5 and the E3 ubiquitin ligase UBRI, and its biological role[J]. Mol Cell, 2006, 24(1):63-75.

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不同剂量⁶⁰Coγ射线照射正常人淋巴母细胞后的差异表达基因分析

200433 上海, 第二军医大学海军医学系放射医学教研究

收稿日期: 2007-09-01

基金项目: 国家自然科学基金资助项目(10575130)

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摘要: 目的研究不同剂量γ射线照射正常人淋巴母细胞AHH-1的基因差异表达,探讨生物学效应的差异。方法 ⁶⁰Coγ射线照射AHH-1,用人cDNA芯片检测照射后8h AHH-1细胞和正常细胞mRNA表达,将芯片分析数据进行聚类分析、判别比较,筛选差异表达基因。结果数据分析筛选后,仅与2.0 Gy照射关系密切的差异表达基因23个;仅在0.5 Gy照射中变化的基因5个;2.0Gy与0.5Gy两组比较变化一致的基因有5个。结论不同剂量γ射线照射AHH-1诱导明显的基因差异表达,其中部分基因表达的改变可能是辐射生物效应发生的关键因素。

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不同剂量60Coγ射线照射正常人淋巴母细胞后的差异表达基因分析

Different gene expression of normal lymphoblastoid cells which exposure to different dose of 60Coγ-ray

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不同剂量60Coγ射线照射正常人淋巴母细胞后的差异表达基因分析

English Abstract

Different gene expression of normal lymphoblastoid cells which exposure to different dose of 60Coγ-ray

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不同剂量⁶⁰Coγ射线照射正常人淋巴母细胞后的差异表达基因分析

Different gene expression of normal lymphoblastoid cells which exposure to different dose of ⁶⁰Coγ-ray

不同剂量⁶⁰Coγ射线照射正常人淋巴母细胞后的差异表达基因分析

Different gene expression of normal lymphoblastoid cells which exposure to different dose of ⁶⁰Coγ-ray