路璐, 李德冠, 王月英, 张俊玲, 吴红英, 孟爱民. 脂多糖对受照小鼠血清IL-10、IL-6和TNF-α水平的影响[J]. 国际放射医学核医学杂志, 2012, 36(3): 154-156. DOI: 10.3760/cma.j.issn.1673-4114.2012.03.008
引用本文: 路璐, 李德冠, 王月英, 张俊玲, 吴红英, 孟爱民. 脂多糖对受照小鼠血清IL-10、IL-6和TNF-α水平的影响[J]. 国际放射医学核医学杂志, 2012, 36(3): 154-156. DOI: 10.3760/cma.j.issn.1673-4114.2012.03.008
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Lu LU, De-guan LI, Yue-ying WANG, Jun-ling ZHANG, Hong-ying WU, Ai-min MENG. Effects of lipopolysaccharide on IL-10, IL-6 and TNF-α levels in radiation-exposed mice[J]. Int J Radiat Med Nucl Med, 2012, 36(3): 154-156. DOI: 10.3760/cma.j.issn.1673-4114.2012.03.008
Citation: Lu LU, De-guan LI, Yue-ying WANG, Jun-ling ZHANG, Hong-ying WU, Ai-min MENG. Effects of lipopolysaccharide on IL-10, IL-6 and TNF-α levels in radiation-exposed mice[J]. Int J Radiat Med Nucl Med, 2012, 36(3): 154-156. DOI: 10.3760/cma.j.issn.1673-4114.2012.03.008
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脂多糖对受照小鼠血清IL-10、IL-6和TNF-α水平的影响

Effects of lipopolysaccharide on IL-10, IL-6 and TNF-α levels in radiation-exposed mice

    摘要
  • 摘要:
    目的 探讨6 Gy 137Cs γ射线一次性全身照射后，小鼠血清中白细胞介素10（IL-10）、白细胞介素6（IL-6）和肿瘤坏死因子α（TNF-α）水平的变化及对脂多糖刺激反应性的远期影响。
    方法 将实验小鼠分为假照射组和照射组，假照射组不接受照射，照射组给予6 Gy γ射线照射。照射后10周，将假照射组和照射组小鼠按组别分别提前24 h和1 h腹腔注射脂多糖（20 mg/kg），对照组注射生理盐水（100 μl/只）。取小鼠外周血，利用酶联免疫吸附技术检测小鼠血清中IL-10、IL-6和TNF-α的水平。
    结果 假照射组小鼠在给予脂多糖刺激1 h后，血清中IL-10、IL-6和TNF-α水平与其对照组相比均显著升高（t=7.31、2.71和15.09，P分别为 < 0.01、 < 0.05和 < 0.01）。照射组小鼠在给予脂多糖刺激1 h后，血清中IL-10、IL-6和TNF-α水平与其对照组相比均显著升高（t=4.14、7.18和5.14，P均 < 0.01）。与假照射组相比，照射组小鼠在给予脂多糖刺激24 h后，TNF-α和IL-6水平无明显变化，IL-10水平升高了19.9%（t=2.84，P < 0.05）。
    结论 经6 Gy γ射线照射后10周，小鼠免疫调节功能尚未完全恢复；在接受脂多糖刺激后，假照射组和照射组小鼠的抗感染能力也有差异。脂多糖对辐射后小鼠血清中IL-10、IL-6和TNF-α水平的影响及其意义仍需进一步研究。

     

    Abstract:
    Objective To investigate the effects of lipopolysaccharide(LPS) on interleukin-10(IL-10), interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) levels in mice exposed whole body to 6 Gy γ-ray radiation once.
    Methods The mice were divided into sham-irradiation group and irradiation group. Mice in sham-irradiation group accepted no irradiation, while mice in irradiation group accepted 6 Gy whole body irradiation at dose rate of 0.8 Gy/min. Mice in LPS treated group accepted intraperitoneal injection of LPS (20 mg/kg)at 10 weeks after irradiation, while mice in control group were accepted intraperitoneal injection of saline. The peripheral blood was collected by eyeballs enucleation after 1 h and 24 h respectively, then IL-10, IL-6 and TNF-α levels were detected by enzyme-linked immunoassay.
    Results Compared to control group, the levels of IL-10, IL-6 and TNF-α in blood serum increased at 1 h and 24 h after LPS injection both in sham-irradiation group(t=7.31, P < 0.01; t=2.71, P < 0.05; t=15.09, P < 0.01) and irradiation group(t=4.14, 7.18 and 5.14, all P < 0.01). Compared to sham-irradiation group, the level of IL-10 increased 19.9% at 24 h after LPS injection in irradiation group(t=2.84, P < 0.05), while the levels of TNF-α and IL-6 had no significant differences between sham-irradiation group and irradiation group.
    Conclusions The immune system of mice had not entirely recovered at 10 weeks after 6 Gy γ-ray irradiation. After LPS injection, there was difference of anti-infection ability between sham-irradiation group and irradiation group. The effects of LPS on cytokines IL-10, IL-6 and TNF-α levels in radiation-exposed mice need to be further explored.

     

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