干细胞移植的磁性标记及磁共振成像活体示踪

周翠屏; 沈君; 梁碧玲

周翠屏 , 沈君 , 梁碧玲

文章导航 > 国际放射医学核医学杂志 > 2006, 30 > 4: (253-256)

引用本文:

Citation:

干细胞移植的磁性标记及磁共振成像活体示踪

510120 广州, 中山大学第二附属医院放射科

通讯作者: 沈君, vencentsj@tom.com ;

基金项目:
广东省自然科学基金博士启动项目(04300241)

国家自然科学基金项目(30400115)
中图分类号: R445.2

Magnetic labelling of transplanted stem cells and in vivo tracking with magnetic resonance imaging

Department of Radiology, Zhongshan University of the Second Affiliated Hospital, Guangzhou 510120, China

Corresponding author: SHEN Jun, vencentsj@tom.com ;

CLC number: R445.2

摘要: 干细胞移植的临床应用需要解决植入活体内干细胞在体内存活、迁移及分化的监测问题。通过对干细胞进行顺磁性标记,磁共振成像(MRI)能够在活体上显示标记的干细胞,并进行特异性地追踪及定位,是目前干细胞活体示踪极具前景的方法。干细胞进行磁性标记主要利用铁类或钆类对比剂,两者各有优缺点。利用铁类或钆类对比剂标记干细胞并进行MRI活体监测取得了成功。并在心脑缺血损伤的疾病模型中得到应用,但在干细胞磁性标记的载体选用及其标记率、标记的持久性、标记对细胞活力及遗传性状方面尚存在一定的问题。
- 造血干细胞移植 /
- 磁共振成像 /
- 磁性标记 /
- 示踪 /
- 活体内
Abstract: How to monitor and track the survival, migration and differentiation of grafted stem cell in vivo is necessary for the widespread clinically application of stem cell transplantation. Manegtic resonance imaging(MRI), which can display and specifically track and localize labelled stem cell by paramagnetically labelling of the stem cell, is an extremely prospective method to monitor stem cell in vivo. The ferrum and gadlinium derviative contrast agents were mainly used to paramagnetically label the stem cell, and each had its own advantages and disadvatages. Currently, on the basis of paramagnetically labelling of the stem cell with ferrum and gadlinium derviative contrast agents, MRI had been succeessfully used to monitor the stem cell in vivo and had been applied into animal model of the heart and brain stroke, but these are still some problems exist such as the selection of vehicle transferring the contrast agents into stem cells, the labeling efficiency, the duration of labelling and the viablity and heredity changes of the stem cell after labelling.

本作品遵循Signature-Non-Commercial Use 4.0 International (CC BY-NC 4.0)协议

[1]	Bulte JW,Douglas T,Witwer B,et al.Magnatodendrimers allow endosomal magnetic labelling and in vivo tracking of stem cells.Nat Biotechnol,2001,19(12):1141-1147.
[2]	Charlotte R,Frank PB,Florence G,et al.Iron oxide nanoparticle labeled rat smooth muscle cells:Cardiac MR imaging for cell graft monitoring and quantitation.Radiology,2005,235(3):959-967.
[3]	Mathias H,Ekkehard KS,James B,et al.Monitoring of implanted stem cell migration in vivo:A highly resolved in vivo magnetic resonance imaging nvestigation of experimental stroke in rat.Proc Natl Acad Sci USA,2002,99(25):162-167.
[4]	Modo M,Mellodew K,Cash D,et al.Mappingtransplanted stem cell migration after a stroke:a serial,in vivo magnetic resonance imaging study.Neuroimage,2004,21(1):311-317.
[5]	Modo M,Cash D,Mellodew K,et al.Tracking transplanted stem cell migration using bifunctional,contrast agent enhanced,magnetic resonance imaging.Neuroimage,2002,17(2):803-811.
[6]	Vuu K,Xie J,McDonald MA,et al.Gadolinium-rhodamine nanoparticles for cell labeling and tracking via magnetic resonance and optical imaging.Bioconjug Chem,2005,16(4):995-999.
[7]	Daldrup-Link HE,Rudelius M,Oostendorp RA,et al.Targeting of hematopoietic progenitor cells with MR contrast agents.Radiology,2003,228(3):760-767.
[8]	Frank JA,Miller BR,Arbab AS,et al.Clinically applicable labelling of mammalian and stem calls by combining superparamagnetic iron oxides and transfection agents.Radiology,2003,228(2):480-487.
[9]	Arbab AS,Yocum GT,Kalish H,et al.Efficient magnetic cell labeling with protamine sulfate complexed toferumoxides for cellular MRI.Blood,2004,104(4):1217-1223.
[10]	Rudelius M,Dald-Link HE,Heinzmann U,et al.Highly efficient paramagnetic labelling of embryonic and neuronal stem cells.Eur J Nucl Med Mol Imaging,2003,30(7):1038-1044.
[11]	Wilhelm C,Billotey C,Roger J,et al.Intracellular uptake of anionicsuperparamagnetic nanoparticles as a function of their surface coating.Biomaterials,2003,24(6):1001-1011.
[12]	居胜红,滕皋军,毛曦.脐血间充质干细胞磁探针标记和MR成像研究.中华放射学杂志,2005,39(1):101-106.
[13]	Hoehn M,Kustermann E,Blunk J,et al.Monitoring of implanted stem cell migration in vivo:a highly resolved in vivo magnetic resonance imaging investigation of experimental stroke in rat.Proc Natl Acad Sci USA,2002,99(25):16267-16272.
[14]	Zhang ZG,Jiang Q,Zhang R,et al.Magnetic resonance imaging and neurosphere therapy of stroke in rat.Ann Neurol,2003,53(2):259-263.
[15]	Kraitchman DL,Heldman AW,Atalar E,et al.In vivo magnetic resonance imaging of mesenchymal stem cells in myocardial infarction.Circulation,2003,107(18):2290-2293.
[16]	Hill JM,Dick AJ,Raman VK,et al.Serial cardiac manetic resonance imaging of injected mesenchymal stem cell.Circulation,2003,108(8):1009-1014.
[17]	Oehn M,Kustermann E,Blunk J,et al.Monitoring of implanted stem cell migration in vivo:a highly resolved in vivo magnetic resonance imaging investigation of experimental stroke in rat.Proc Natl Acad Sci USA,2002,99(25):16267-16272.

[1]	王静 , 姚振威 . 细胞磁共振成像技术在体示踪免疫细胞的研究进展. 国际放射医学核医学杂志, 2014, 38(5): 337-340. doi: 10.3760/cma.j.issn.1673-4114.2014.05.014
[2]	樊鹏 , 甄俊平 , 王峻 . MR示踪技术在干细胞移植治疗软骨缺损中的研究进展. 国际放射医学核医学杂志, 2015, 39(6): 497-500. doi: 10.3760/cma.j.issn.1673-4114.2015.06.013
[3]	万绪明 , 许祖闪 , 侯红军 . 磁共振表观弥散系数变化率对肝细胞肝癌患者单次TACE术后疗效评价的应用价值. 国际放射医学核医学杂志, 2015, 39(2): 129-135. doi: 10.3760/cma.j.issn.1673-4114.2015.02.006
[4]	闵捷 , 李斌 , 金凯 , 梁维仁 , 晁明 . 肝脏磁共振弹性成像的应用现状与展望. 国际放射医学核医学杂志, 2014, 38(4): 261-265. doi: 10.3760/cma.j.issn.1673-4114.2014.04.013
[5]	朱灿胜 , 胡学强 . 超小超顺磁性氧化铁粒子标记免疫细胞在多发性硬化症动物模型MRI中的应用. 国际放射医学核医学杂志, 2006, 30(5): 312-314.
[6]	张华 , 姚振威 . 细胞示踪影像学技术的发展. 国际放射医学核医学杂志, 2015, 39(2): 180-183. doi: 10.3760/cma.j.issn.1673-4114.2015.02.017
[7]	叶根耀 . 核辐射事故的医学处理新进展. 国际放射医学核医学杂志, 2003, 27(3): 123-127.
[8]	尹培 , 边艳珠 . 体内凋亡细胞显像的研究进展. 国际放射医学核医学杂志, 2009, 33(5): 279-282. doi: 10.3760/cnla.j.issn.1673-4114.2009.05.007
[9]	董庆玉 , 陈丽 . 骨髓间充质干细胞的活体标记示踪技术及其研究进展. 国际放射医学核医学杂志, 2007, 31(5): 314-317.
[10]	田海静 . 磁共振心肌灌注成像对急性心肌梗死的临床评价. 国际放射医学核医学杂志, 2007, 31(6): 388-388.

点击查看大图

计量

文章访问数: 1367
HTML全文浏览量: 88
PDF下载量: 2

干细胞移植的磁性标记及磁共振成像活体示踪

通讯作者: 沈君, vencentsj@tom.com;

510120 广州, 中山大学第二附属医院放射科

收稿日期: 2005-12-27

基金项目: 广东省自然科学基金博士启动项目(04300241)国家自然科学基金项目(30400115)

关键词:

摘要: 干细胞移植的临床应用需要解决植入活体内干细胞在体内存活、迁移及分化的监测问题。通过对干细胞进行顺磁性标记,磁共振成像(MRI)能够在活体上显示标记的干细胞,并进行特异性地追踪及定位,是目前干细胞活体示踪极具前景的方法。干细胞进行磁性标记主要利用铁类或钆类对比剂,两者各有优缺点。利用铁类或钆类对比剂标记干细胞并进行MRI活体监测取得了成功。并在心脑缺血损伤的疾病模型中得到应用,但在干细胞磁性标记的载体选用及其标记率、标记的持久性、标记对细胞活力及遗传性状方面尚存在一定的问题。