人外周血白细胞黏附分子的辐射效应研究
Research on effects of ionizing radiation of human peripheral blood white cell adhesive molecules
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摘要: 目的 研究外周血白细胞表面黏附分子的表达及其介导的细胞黏附能力变化与辐射剂量的关系。方法 人外周血细胞经不同剂量照射后不同时间用流式细胞仪双色标记分析不同白细胞表面不同黏附分子表达,特异底物包被微孔板法和结晶紫染色分析检测单核细胞对不同底物的黏附能力。结果 单核细胞表面CD11b和粒细胞表面CD29表达下降与辐射剂量间存在良好量效关系,照射后单核细胞对底物β1-整合素和胶原蛋白I的黏附能力改变与辐射剂量存在一定量效关系。结论 外周血白细胞表面黏附分子表达及功能改变展现出的与辐射剂量间的良好关系,为进一步深入研究将其作为评估生物受照剂量指标的可能打下基础。Abstract: Objective To investigate the links between expression and function of adhesive molecule on the surface of irradiated peripheral blood white cells.Methods Heparinized human peripheral blood was exposed to γ rays with different dose.At the different post-radiation time adhesive molecule expression on cellular surface was determined by double fluorescence labeling antibodies which were against adhesive molecule and special mark of granulocyte or mononuclear cell respectively with flow cytometry,and ceUular adhesive ability to different matrixes mediated by adhesive molecule WaS estimated by commercializing enzyme-linked inununosorbent assay kit and crystalviolet dying.Results A decline pattern of CD11b on surface ofmononuelear cells and CD29 on surface of granulocyte with irradiation dose increase was found.The changes of adhesive ability of mononuclear ceHs to substance of β1-integrin and collagen-I Was well related with irradiation dose.Conclusion Good relationship shown by the changes of adhesive molecule expression and adhesive ability mediated by the molecules on the surface of peripheral blood white cells with radiation dose was primary base of further research on indicting exposure dose by biomarker.
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Key words:
- Dose-response relationship /
- radiation /
- Leukocyte /
- Cell adhesion molecule /
- Cell adhesion
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