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各种放疗、化疗治疗肿瘤的主要作用机制之一是干扰肿瘤细胞DNA合成和抑制细胞分裂,诱导其发生凋亡,最终达到抑制或消除肿瘤的目的。1972年,Kerr等[1]科学家提出了细胞凋亡的概念。测定肿瘤细胞凋亡成为早期评价肿瘤疗效的指标之一。目前检测细胞凋亡的方法分为体外、体内两大类。体外检测技术开展较早,发展较成熟,但均属有创检查,并且通常只能在某一时间点进行凋亡检测,不能连续动态监测细胞凋亡在活体内的发生和发展过程,临床实际应用价值有限[2]。
放射性核素凋亡显像是目前研究最为广泛、技术最为成熟的体内细胞凋亡分子影像学检测技术。在肿瘤细胞凋亡领域,特异性分子探针的研发是显像的关键技术之一,其主要包括单光子显像剂和正电子显像剂,分别应用于SPECT和PET。
肿瘤细胞凋亡核素显像分子探针研究进展
Progress in molecular probes of radionuclide tumor apoptosis imaging
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摘要: 测定肿瘤细胞凋亡是早期评价肿瘤疗效的指标之一, 放射性核素凋亡显像是目前研究最为广泛、技术最为成熟的体内肿瘤细胞凋亡分子影像学检测技术, 能在活体内动态、无创地检测抗肿瘤治疗引起的细胞凋亡, 有助于肿瘤疗效的早期评判和预后分析, 其中特异性分子探针技术的研究开发是放射性核素凋亡显像的关键技术之一。笔者将目前有代表性的肿瘤细胞凋亡核素显像分子探针进行了总结。Abstract: Apoptosis is one of the important indices about the early assessment of the efficacy of tumor treatment. Among molecular imaging techniques of tumor apoptosis, radionuclide imaging is the most extensively studied and the most sensitive imaging modality, which can noninvasively and dynamically detect cell apoptosis induced by treatment in vivo, especially for efficacy of cancer therapies and prognosis of malignancies. Recently, as one of the key techniques, specific molecular probes are being developed. The representative molecular probes of the radionuclide imaging for tumor apoptosis are reviewed.
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Key words:
- Radionuclide imaging /
- Neolpasms /
- Apoptosis
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