-
核能的迅速发展及核技术在工业、农业、军事、医疗等各个领域被广泛应用,已取得的社会效益和经济效益令人瞩目,然而由于操作不当、管理不善或者其他原因,核与辐射事故时有发生,会造成急性辐射损伤,利用干细胞修复损伤细胞,是治疗急性辐射损伤的一个重要途径。间充质干细胞(mesenchymal stem cells,MSC)具有易于外源基因转染和表达、能分泌造血因子、抗原性小、组织修复能力强等特征。目前,MSC已广泛应用在急性心肌梗死[1]、炎症性肠病[2]、急性肝衰竭[3]、血液系统疾病[4]、脊髓损伤[5]、骨缺损[6]、自身免疫性疾病[7]等临床治疗中,同时也可用于治疗辐射损伤所致的多脏器功能不全[8]。
应用间充质干细胞治疗急性辐射损伤的研究现状与进展
Recent advances in the application of mesenchymal stem cells for treatment of acute radiation injury
-
摘要: 间充质干细胞是一类来源广泛的低免疫原性多能干细胞, 具有多向分化、造血支持、免疫调控等特点, 且易于分离、扩增和外源基因转染, 在临床治疗中有广泛的应用价值, 其用于急性放射损伤治疗的报道也较多。笔者主要介绍了间充质干细胞治疗急性放射损伤的研究现状和相关进展。Abstract: Mesenchymal stem cells(MSCs) are a source of various multipotent stromal cells with low inherent immunogenicity.These cells can undergo multi-directional differentiation, support hematopoiesis, and regulate immune responses.MSCs can be easily collected and amplified.These cells can also be transfected using an exogenous gene.MSCs have been extensively applied in clinical treatments, such as in the therapy for acute radiation injury.This study was primarily performed to investigate the effect and mechanism of MSCs in acute radiation injury.
-
Key words:
- Mensenchymal stem cell /
- Repair /
- Radiation injury
-
[1] Santos ND, Mosqueira D, Sousa LM, et al.Human umbilical cord tissuederived mesenchymal stromal cells attenuate remodeling after myocardial infarction by proangiogenic, antiapoptotic, and endogenous cell-activation mechanisms[J].Stem Cell Res Ther, 2014, 5(1):155-163.DOI:10.1186/scrt394. [2] Sémont A, Demarquay C, Bessout R, et al.Mesenchymal stem cell therapy stimulates endogenous host progenitor cells to improve colonic epithelial regeneration[J/OL].PLoS One, 2013, 8(7): e70170.[2015-10-10].http://www.medsci.cn/sci/show_paper.asp?id=204e5463685.DOI:10.1371/journal.pone.0070170. [3] Liu ZC, Meng FW, Li C, et al.Human umbilical cord mesenchymal stromal cells rescue mice from acetaminophen-induced acute liver failure[J].Cytotherapy, 2014, 16(9):1207-1219.DOI:10.1016/j.jcyt.2014.05.018. [4] Tripathy NK, Singh SP, Nityanand S, et al.Enhanced adipogenicity of bone marrow mesenchymal stem cells in aplastic anemia[J/OL].2014, 2014: 276862.[2015-10-10].http://www.hindawi.com/journals/sci/2014/276862.DOI:10.1155/2015/276862. [5] Liu J, Han DM, Wang ZD, et al.Clinical analysis of the treatment of spinal cord injury with umbilical cord mesenchymal stem cells[J].Cytotherapy, 2013, 15(2):185-191.DOI:10.1016/j.jcyt.2012.09.005. [6] Mardones R, JofréCM, Minquell JJ, et al.Cell therapy and tissue engineering approaches for cartilage repair and/or regeneration[J].Inter Stem Cells, 2015, 8(1):48-53.DOI:10.15283/ijsc.2015.8.1.48. [7] Luk F, De Witte SF, Bramer WM, et al.Efficacy of immunotherapy with mesenchymal stem cells in man:a systematic review[J].Expert Rev Clin Immunol, 2015, 11(5):617-636.DOI:10.1586/1744666X.2015.1029458. [8] Chapel A, Francois S, Douay L, et al.Fifteen years of preclinical and clinical experiences about biotherapy treatment of lesions induced by accidental irradiation and radiotherapy[J].World J Stem Cells, 2013, 5(3):68-72.DOI:10.4252/wjsc.v5.i3.68. [9] Chua HL, Plett PA, Sampson CH, et al.Long-term hematopoietic stem cell damage in a murine model of the hematopoietic syndrome of the acute radiation syndrome[J].Health Phys, 2012, 103(4):356-366.DOI:10.1097/HP.0b013e3182666d6f. [10] Hu KX, Sun QY, Guo M, et al.The radiation protection and therapy effects of mesenchymal stem cells in mice with acute radiation injury[J].Bri J Radiol, 2010, 83(985):52-58.DOI:10.1259/bjr/61042310. [11] 胡锴勋, 赵士富, 郭梅, 等.间充质干细胞对小鼠辐射早期造血组织细胞的细胞周期及凋亡的影响[J].中国实验血液学杂志, 2007, 15(6):1226-1230.DOI:10.3969/j.issn.1009-2137.2007.06.021.
Hu KX, Zhao SF, Guo M, et al.Effects of mesenchymal stem cells on cell cycle and apoptosis of hematopoietic tissue cells in irrdiated mice[J].J Exp Hematol, 2007, 15(6):1226-1230. doi: 10.3969/j.issn.1009-2137.2007.06.021[12] Shim S, Lee SB, Lee JG, et al.Mitigating effects of hUCB-MSCs on the hematopoietic syndrome resulting from total boby irradiation[J].Exp Hematol, 2013, 41(4):346-353, e2.DOI:10.1016/j.exphem.2013.01.002. [13] 胡佳乐, 徐立军, 徐之明, 等.人骨髓间充质干细胞移植对辐射NOD/SCID小鼠造血重建的影响[J].实用癌症杂志, 2013, 28(2):111-113.DOI:10.3969/j.issn.1001-5930.2013.02.001.
Hu JL, Xu LJ, Xu ZM, et al.Effect of human bone marrow mesenchymal stem cells transplantation on NOD/SCID mouse hematopoietic reconstruction)[J].Pract J Cancer, 2013, 28(2):111-113. doi: 10.3969/j.issn.1001-5930.2013.02.001[14] Wang Y, Chen XD, Cao W, et al.Plasticity of mesenchymal stem cells in immunomodulation:pathological and therapeutic implications[J].Nat Immunol, 2014, 15(11):1009-1016.DOI:10.1038/ni.3002. [15] 艾辉胜, 余长林, 乔建辉, 等.山东济宁Co60辐射事故受照人员的临床救治疗[J].中华放射医学与防护杂志, 2007, 27(1):1-5.
Ai HS, Yu CL, Qiao JH, et al.Medical management of irradiated patlents in a radiation accident in Jining Shangong Province[J].Chin J Radiol Med Protect, 2007, 27(1):1-5.[16] Baron F, Lechanteur C, Willeas E, et al.Cotransplantaion of mesenchymal stem cells might prevent death from graft-versus-hose disease(GVHD) without abrogating graft-versus-tumor effects after HLA-mismatched allogeneic transplantation following nonmyeloablative conditioning[J].Biol Blood Marrow Transplant, 2010, 16(6):838-847.DOI:10.1016/j.bbmt.2010.01.011. [17] 胡锴勋, 赵士富, 郭梅, 等.骨髓间充质干细胞防治多脏器衰竭[J].中华急诊医学杂志, 2009, 18(6):607-610.DOI:10.3760/cma.j.issn.1671-0282.2009.06.014.
Hu KX, Zhao SF, Guo M, et al.Mesenchymal stem cells protecting multiple organ failure[J].Chin J Emerg Med, 2009, 18(6):607-610. doi: 10.3760/cma.j.issn.1671-0282.2009.06.014[18] Qiao S, Ren H, Shi Y, et al.Allogeneic compact bone-derived mesenchymal stem cell transplantation increases survival of mice exposed to lethal total body irradiation:a potential immunological mechanism[J].Chin Med J(Engl), 2014, 127(3):475-482.DOI:10.3760/cma.j.issn.0366-6999.20132001. [19] Chen H, Min XH, Wang QY, et al.Pre-activation of mesenchymal stem cells with TNF-alpha, IL-1 beta and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury[J].Sci Rep, 2015, 5(5):1-14.DOI:10.1038/srep08718. [20] Okamoto R, Yajima T, Yamazaki M, et al.Damaged epithelia regenerated by bone marrow-derived cells in the human gastrointestinal tract[J].Nat Med, 2002, 8(9):1011-1017.DOI:10.1038/nm755. [21] Semont A, Francois S, Mouiseddine M, et al.Mesenchymal stem cells increase self-renewal of small intestinal epithelium and accelerate structural recovery after radiation jnjury[J].Adv Exp Med Biol, 2006, 585:19-30.DOI:10.1007/978-0-387-34133-0_2. [22] Saha S, Bhanja P, Kabarriti R, et al.Bone marrow stromal cell transplantation mitigates radiation-induced gastrointestinal syndrome in mice[J/OL].PLoS One, 2011, 6(9): e24072[2015-10-10].http://www.ncbi.nlm.nih.gov/pubmed/21935373?dopt=Abstract.DOI:10.1371/journal.pone.0024072. [23] Devine SM, Cobbs C, Jennings M, et al.Mesenchymal stem cells distribute to a wide range of tissues following systemic infusion into nonhuman primates[J].Blood, 2003, 101(8):2999-3001.DOI:10.1182/blood-2002-06-1830. [24] Guo J, Lin GS, Bao CY, et al.Anti-inflammation role for mesenchymal stem cells transplantation in myocardial infarction[J].Inflammation, 2007, 30(3-4):97-104.DOI:10.1007/s10753-007-9025-3. [25] Zhang J, Gong JF, Zhang W, et al.Effects of transplanted bone marrow mesenchymal stem cells on the irradiated intestine of mice[J].J Biomed Sci, 2008, 15(5):585-594.DOI:10.1007/s11373-008-9256-9. [26] Herzog EL, Chai L, Krause DS.Plasticity of marrow-derived stem cells[J].Blood, 2003, 102(10):3483-3493.DOI:10.1182/blood-2003-05-1664. [27] 朱红燕, 张宏, 傅晋翔, 等.骨髓间充质干细胞与急性皮肤放射损伤的修复[J].中国组织工程研究与临床康复, 2009, 13(32):6303-6308.DOI:10.3969/j.issn.1673-8225.2009.32.020.
Zhu HY, Zhang H, Fu JX, et al.Bone marrow mesenchymal stem cells promote the regeneration of irradiation-induced acute skin injury[J].J Clinical Rehabilitative Tissue Engineering Res, 2009, 13(32):6303-6308. doi: 10.3969/j.issn.1673-8225.2009.32.020[28] 刘晓玉, 王瑞, 张涛, 等.人脂肪间充质干细胞对皮肤创伤修复的作用[J].基础医学与临床, 2013, 33(11):1377-1381.
Liu XY, Wang R, Zhang T, et al.Effects of human adipose-derived mesenchymal stem cells on cutaneous wound healing[J].Basic Med Sci Clin, 2013, 33(11):1377-1381.[29] Lataillade JJ, Doucet C, Bey E, et al.New approach to radiation bum treatment by dosimetry-guided surgery combined with autologous mesenchymal stem cell therapy[J].Regen Med, 2007, 2(5):785-794.DOI:10.2217/17460751.2.5.785. [30] 王东平, 刘爱兵, 周立冬.脐带间充质干细胞治疗放射性皮肤损伤[J].中国美容医学, 2012, 21(16):67-68.DOI:10.3969/j.issn.1008-6455.2012.16.052.
Wang DP, Liu AB, Zhou LD.Mesenchymal stem cells therapy for radio-skin lesion[J].Chin J Aesthet Med, 2012, 21(16):67-68. doi: 10.3969/j.issn.1008-6455.2012.16.052[31] 王涛, 廖天安, 王鸿, 等.血管内皮生长因子基因修饰骨髓间充质干细胞移植于放射治疗后组织的实验研究[J].国际口腔医学杂志, 2014, 41(2):133-136.DOI:10.7518/gjkq.2014.02.003.
Wang T, Liao TA, Wang H, et al.Transplantation of vascular endothelial growth factor gene-modified bone mesenchymal stem cells into irradiated tissue[J].Inte J Stomatol, 2014, 41(2):133-136. doi: 10.7518/gjkq.2014.02.003[32] Gan J, Meng F, Zhou X, et al.Hematopoietic recovery of acute radiation syndrome by human superoxide dismutase-expressing umbilical cord mesenchymal stromal cells[J].Cytotherapy, 2015, 17(4):403-417.DOI:10.1016/j.jcyt.2014.11.011. [33] 臧宇, 陈伟, 徐开林, 等.CXCR4基因修饰骨髓间充质干细胞对照射损伤小鼠造血重建的影响[J].中国实验血液学杂志, 2013, 21(5):1261-1265.DOI:10.7534/j.issn.1009-2137.2013.05.035.
Zang Y, Chen W, Xu KL, et al.Influence of CXCR4 overexpressed mesenchymal stem cells on hematopoietic recovery of irradiated mice[J].J Exp Hematol, 2013, 21(5):1261-1265. doi: 10.7534/j.issn.1009-2137.2013.05.035
计量
- 文章访问数: 3067
- HTML全文浏览量: 1984
- PDF下载量: 4