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在大范围的核辐射事故或核攻击事件中,运用辐射生物剂量估算对患者进行成功地分类诊断及合理治疗显得非常重要。染色体畸变分析方法是目前生物剂量估算的金标准,但是这种方法需要进行细胞培养,受照后3 d才能给出准确的结果,并且还需要有经验丰富的细胞遗传学技术人员来操作。因此需要发展新的高通量、自动化且经济的生物剂量估算方法。基因芯片技术是一种信息量大、操作简单、速度快捷的核酸序列测定及定量分析技术,已广泛应用于放射生物学的研究中。辐射所造成的分子水平基因调控是目前研究的热点,由于外周血具有易采集、辐射响应迅速等优点,因此大多数研究的研究对象是外周血细胞,尤其是外周血淋巴细胞(peripheral blood lymphocyte,PBL)在转录水平的变化。现将应用基因芯片技术筛选辐射响应基因及利用筛选基因表达信号建立剂量评价模型等的研究现状综述如下。
外周血核基因表达评价辐射生物剂量方法研究进展
Advances of nuclear gene expression signatures in peripheral blood for irradiation biodose estimation
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摘要: 基因芯片技术可以完整地了解辐射引起的细胞或组织差异基因表达变化,已广泛应用于放射生物学的研究。外周血有核细胞,特别是淋巴细胞对辐射非常敏感,全面了解辐射后外周血有核细胞基因表达变化,对于辐射生物剂量分子指标研究及建立剂量评价模型非常重要。该文总结了目前利用基因芯片技术筛选外周血辐射响应基因,并利用筛选基因表达信号建立评价模型等的研究现状。Abstract: As gene chip technology helps get a more comprehensive understanding of the differentially expressed genes in cells or tissues induced by ionizing radiation, it has been widely used in the research of radiobiology to screen biomarkers. The peripheral blood nucleated cells are extremely sensitive to ionizing radiation, especially lymphocytes. Therefore, it is important to better understand the gene expression changes of peripheral blood nucleated cells for studying molecular biomarkers of ionizing radiation biodosimetry and building the prediction model to estimate radiation exposures. The paper briefly describes the latest research progress in screening ionizing radiation response genes from peripheral blood by means of gene chip technology as well as building the prediction model using expression changes of screened genes.
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Key words:
- Ionizing radiation /
- Biological dose /
- Gene chip /
- Gene Expression
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