-
放射疗法是治疗现代肿瘤的主要手段之一,约70%的肿瘤患者需要接受放疗[1]。但是与几十年前相比,肿瘤的治愈率并没有明显提高[2]。辐射增敏剂一直是放射肿瘤治疗学研究的重大课题[3]。辐射增敏实验需要对荷瘤鼠的肿瘤进行照射,然后观测肿瘤生长情况。为了使肿瘤照射实验简单、易行,防止部分肿瘤漏照,我们对肿瘤照射实验动物模型进行了改进。
实验肿瘤局部照射动物模型的改进
Improvement on the animal model of local irradiation for experimental tumors
-
摘要:
目的 对局部照射肿瘤实验动物模型进行改进以提高肿瘤放疗增敏药物增敏实验结果的可靠性。 方法 分别在小鼠腿上皮下(传统方法)和小鼠脚背皮下(改进方法)接种肝癌H22细胞,使其生长成实体瘤;用5 Gy γ射线照射肿瘤后,每隔1 d测量肿瘤的长、宽、高并计算肿瘤体积,连续观察24 d。 结果 将肿瘤接种于小鼠腿上皮下时,照射组的肿瘤体积和质量与对照组之间的差异无统计学意义(t=0.55、0.70,P均>0.05);而将肿瘤接种于脚背皮下时,照射组的肿瘤体积和质量均明显低于对照组(t=2.25,P < 0.05;t=3.14,P < 0.01)。 结论 肿瘤接种于小鼠脚背皮下的方法,操作简单、方便、易行且实验结果准确、可靠。 Abstract:Objective To improve the reliability of experimental outcome in radio-sensitive drug research by improving the animal model of locally irradiated tumor. Methods Liver cancer cells H22 were implanted into legs and insteps subcutaneously in mice, respectively, and made them grow into solid tumors. Then the tumors were treated with the γ ray of 137Cs at the dosage of 5 Gy. Measured length, width and height of tumors once per day, and calculated volume of tumors, observed for 24 days. Results Volume and weight of the irradiated tumor inoculated into legs were no obvious difference(t=0.55、0.70, both P>0.05). But volume and weight of the irradiated tumors inoculated subcutaneously into insteps of the tested mice were obviously lower than that of control mice(t=2.25, P < 0.05 in volume; t=3.14, P < 0.01 in weight). Conclusion The insteps subcutaneous approach of tumors in mice is a more simple, convenient and practicable operation, and will make the testing result more accurate and reliabe. -
Key words:
- Liver neoplasms /
- Radiation injuries, experimental /
- Models, animal
-
[1] Baskar R, Lee KA, Yeo R, et al. Cancer and radiation therapy: current advances and future directions. Int J Med Sci, 2012, 9(3): 193-199. [2] James ND, Hussain SA, Hall E, et al. Radiotherapy with or without chemotherapy in muscle-invasive bladder cancer. N Engl J Med, 2012, 366(16): 1477-1488. [3] Morgan MA, Parsels LA, Maybaum J, et al. Improving gemcitabine-mediated radiosensitization using molecularly targeted therapy: a review. Clin Cancer Res, 2008, 14(21): 6744-6750. [4] 李仪奎, 王钦茂, 周金黄, 等.中药药理实验方法学.上海:上海科学技术出版社, 1991: 512-513.
[5] Zhang X, Yang H, Gu K, et al. In vitro and in vivo study of a nanoliposomal cisplatin as a radiosensitizer[J/OL]. Int J Nanomedicine, 2011, 6: 437-444[2012-10-11]. http://www.dovepress.com/in-vitro-and-in-vivo-study-of-a-nanoliposomal-cisplatin-as-a-radiosens-peer-reviewed-article-IJN. [6] 周杰, 漆松涛, 潘军, 等.人颅咽管瘤裸鼠皮下移植瘤模型的初步建立.重庆医学, 2012, 41(5): 455-457. doi: 10.3969/j.issn.1671-8348.2012.05.015
[7] 屠珏, 陈方明, 周卫民, 等.不同方法建立的人大细胞肺癌裸鼠移植瘤模型的生物学特点.中国比较医学杂志, 2012, 22(4): 58-67. doi: 10.3969/j.issn.1671.7856.2012.04.014