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免疫系统对电离辐射极为敏感,机体受到较高剂量的电离辐射会引起免疫系统损伤和炎症反应。多种细胞因子作为重要的炎症介质和免疫调节因子,在免疫系统损伤的调节中发挥着重要的作用。本研究分析小鼠接受亚致死剂量6 Gy 137Cs γ射线全身照射后10周,利用炎症免疫刺激剂脂多糖进行攻击,小鼠血液中抗炎性细胞因子白细胞介素10(interleukin-10,IL-10)、白细胞介素6(interleukin-6,IL-6)和肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)水平的变化,以探讨脂多糖刺激对小鼠血清中IL-10、IL-6和TNF-α水平的影响。
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6 Gy γ射线照射后10周,各组小鼠的体质量见表 1。假照射组和照射组小鼠体质量之间差异无统计学意义(t=0.43、0.47和1.61,P均 > 0.05),说明各组小鼠的身体状态没有差异。
组别 假照射组 照射组 对照组 26.62±1.15 26.32±1.03 脂多糖员h组 24.34±1.07 23.55±3.61 脂多糖24 h组 24.86±1.41 21.73±4.11 表 1 6 Gy γ射线全身照射后10周假照射组和照射组小鼠的体质量
(x±s,g) -
6 Gy γ射线照射后10周,各组小鼠血清中IL-10、IL-6和TNF-α的水平见表 2、表 3和表 4。假照射组小鼠在给予脂多糖刺激1 h后,血清中IL-10、IL-6和TNF-α水平与其对照组相比均显著升高(t=7.31、2.71和15.09,P分别为 < 0.01、 < 0.05和 < 0.01),照射组小鼠在给予脂多糖刺激1 h后,血清中IL-10、IL-6和TNF-α水平与其对照组相比均显著升高(t=4.14、7.18和5.14,P均 < 0.01),假照射组小鼠在给予脂多糖刺激24 h后,血清中IL-10和IL-6水平与其对照组相比均显著升高(t=5.37和6.69,P < 0.01)。脂多糖刺激1h后,与假照射组相比,照射组小鼠血清中IL-10水平升高了76.5%(t=2.61,P < 0.05)、IL-6水平升高了4.1%(t=2.55,P < 0.05)。脂多糖刺激24 h后,与假照射组相比,照射组小鼠血清中IL-6和TNF-α水平无显著变化,IL-10水平升高了19.9%(t=2.84,P < 0.05)。
组别 假照射组 照射组 对照组 9.22±0.80 8.62±0.58 脂多糖员h组 15.14±1.63 26.73±9.58 脂多糖24 h组 14.80±2.18 17.75±0.81 表 2 6 Gy γ射线全身照射后10周小鼠血清中白细胞介素10的水平
(x±s,pg/ml) 组别 假照射组 照射组 对照组 26.04±0.63 26.09±0.71 脂多糖员h组 27.42±0.94 28.54±0.28 脂多糖24 h组 34.74±2.84 33.04±7.38 表 3 6 Gy γ射线全身照射后10周小鼠血清中白细胞介素6的水平
(x±s,pg/ml) 组别 假照射组 照射组 对照组 63.73±2.01 74.49±3.76 脂多糖员h组 1128.17±157.74 840.23±332.92 脂多糖24 h组 99.43±9.71 86.35±20.32 表 4 6 Gy γ射线全身照射后10周小鼠血清中肿瘤坏死因子α的水平
(x±s,pg/ml)
脂多糖对受照小鼠血清IL-10、IL-6和TNF-α水平的影响
Effects of lipopolysaccharide on IL-10, IL-6 and TNF-α levels in radiation-exposed mice
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摘要:
目的 探讨6 Gy 137Cs γ射线一次性全身照射后,小鼠血清中白细胞介素10(IL-10)、白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)水平的变化及对脂多糖刺激反应性的远期影响。 方法 将实验小鼠分为假照射组和照射组,假照射组不接受照射,照射组给予6 Gy γ射线照射。照射后10周,将假照射组和照射组小鼠按组别分别提前24 h和1 h腹腔注射脂多糖(20 mg/kg),对照组注射生理盐水(100 μl/只)。取小鼠外周血,利用酶联免疫吸附技术检测小鼠血清中IL-10、IL-6和TNF-α的水平。 结果 假照射组小鼠在给予脂多糖刺激1 h后,血清中IL-10、IL-6和TNF-α水平与其对照组相比均显著升高(t=7.31、2.71和15.09,P分别为 < 0.01、 < 0.05和 < 0.01)。照射组小鼠在给予脂多糖刺激1 h后,血清中IL-10、IL-6和TNF-α水平与其对照组相比均显著升高(t=4.14、7.18和5.14,P均 < 0.01)。与假照射组相比,照射组小鼠在给予脂多糖刺激24 h后,TNF-α和IL-6水平无明显变化,IL-10水平升高了19.9%(t=2.84,P < 0.05)。 结论 经6 Gy γ射线照射后10周,小鼠免疫调节功能尚未完全恢复;在接受脂多糖刺激后,假照射组和照射组小鼠的抗感染能力也有差异。脂多糖对辐射后小鼠血清中IL-10、IL-6和TNF-α水平的影响及其意义仍需进一步研究。 Abstract:Objective To investigate the effects of lipopolysaccharide(LPS) on interleukin-10(IL-10), interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) levels in mice exposed whole body to 6 Gy γ-ray radiation once. Methods The mice were divided into sham-irradiation group and irradiation group. Mice in sham-irradiation group accepted no irradiation, while mice in irradiation group accepted 6 Gy whole body irradiation at dose rate of 0.8 Gy/min. Mice in LPS treated group accepted intraperitoneal injection of LPS (20 mg/kg)at 10 weeks after irradiation, while mice in control group were accepted intraperitoneal injection of saline. The peripheral blood was collected by eyeballs enucleation after 1 h and 24 h respectively, then IL-10, IL-6 and TNF-α levels were detected by enzyme-linked immunoassay. Results Compared to control group, the levels of IL-10, IL-6 and TNF-α in blood serum increased at 1 h and 24 h after LPS injection both in sham-irradiation group(t=7.31, P < 0.01; t=2.71, P < 0.05; t=15.09, P < 0.01) and irradiation group(t=4.14, 7.18 and 5.14, all P < 0.01). Compared to sham-irradiation group, the level of IL-10 increased 19.9% at 24 h after LPS injection in irradiation group(t=2.84, P < 0.05), while the levels of TNF-α and IL-6 had no significant differences between sham-irradiation group and irradiation group. Conclusions The immune system of mice had not entirely recovered at 10 weeks after 6 Gy γ-ray irradiation. After LPS injection, there was difference of anti-infection ability between sham-irradiation group and irradiation group. The effects of LPS on cytokines IL-10, IL-6 and TNF-α levels in radiation-exposed mice need to be further explored. -
Key words:
- Lipopolysaccharides /
- Gamma rays /
- Interleukin-10 /
- Interleukin-6 /
- Tumor necrosis factor-alpha
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表 1 6 Gy γ射线全身照射后10周假照射组和照射组小鼠的体质量
(x±s,g) 组别 假照射组 照射组 对照组 26.62±1.15 26.32±1.03 脂多糖员h组 24.34±1.07 23.55±3.61 脂多糖24 h组 24.86±1.41 21.73±4.11 表 2 6 Gy γ射线全身照射后10周小鼠血清中白细胞介素10的水平
(x±s,pg/ml) 组别 假照射组 照射组 对照组 9.22±0.80 8.62±0.58 脂多糖员h组 15.14±1.63 26.73±9.58 脂多糖24 h组 14.80±2.18 17.75±0.81 表 3 6 Gy γ射线全身照射后10周小鼠血清中白细胞介素6的水平
(x±s,pg/ml) 组别 假照射组 照射组 对照组 26.04±0.63 26.09±0.71 脂多糖员h组 27.42±0.94 28.54±0.28 脂多糖24 h组 34.74±2.84 33.04±7.38 表 4 6 Gy γ射线全身照射后10周小鼠血清中肿瘤坏死因子α的水平
(x±s,pg/ml) 组别 假照射组 照射组 对照组 63.73±2.01 74.49±3.76 脂多糖员h组 1128.17±157.74 840.23±332.92 脂多糖24 h组 99.43±9.71 86.35±20.32 -
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