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恶性肿瘤新陈代谢速度加快,就需要新生血管提供充足的氧和营养物质来满足肿瘤生存及进展的需要。目前,抗血管疗法已成为一种常用的临床治疗恶性肿瘤的方法[1]。本研究用新西兰大白兔制作的VX2肺肿瘤模型作为实验对象,对其进行11C-胆碱、18F-FDG PET-CT对比研究,并与病理及免疫组化结果对照,以检验11C-胆碱、18F-FDG PET-CT反映肿瘤血管生成的效能。
兔VX2肺肿瘤PET-CT与血管生成的相关性研究
The correlation between PET-CT imaging and microvessed density in rabbit lung VX2 tumor model
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摘要:
目的 探讨11C-胆碱和18F-FDG PET-CT反映兔VX2肺肿瘤血管生成的效能。 方法 新西兰大白兔54只,右肺接种VX2肿瘤10~11 d后,行11C-胆碱和18F-FDG PET-CT,测量肿瘤11C-胆碱和18F-FDG最大标准化摄取值(SUVmax)。同时,测量肿瘤标本大小,用免疫组化方法评价肿瘤微血管密度(MVD)。然后对肿瘤胆碱SUVmax、18F-FDG SUVmax与肿瘤大小、MVD进行相关性分析。 结果 33只实验兔成功完成所有检查,得到VX2肺肿瘤的11C-胆碱SUVmax平均值为4.02±3.07 (1.4~12.2),对18F-FDG的SUVmax平均值为5.70±3.45 (1.0~13.0),肿瘤大小平均值为(1.68±1.61)cm3 (0.13~8.00 cm3)。高倍镜(200倍, 0.739 mm2)视野下,肿瘤MVD平均值为(35.8±13.6)个(13~64个)。经统计学分析,11C-胆碱SUVmax与肿瘤大小及MVD之间均不具有相关性;18F-FDG SUVmax与MVD (r=0.525, P=0.002)之间存在正相关关系,与肿瘤大小(r=0.335, P=0.057)之间呈临界正相关关系。 结论 18F-FDG PET-CT可反映兔VX2肺肿瘤血管生成,11C-胆碱PET-CT不能反映肿瘤血管生成。 Abstract:Objective To evaluate and compare the suitability of 11C-choline and 18F-FDG PET-CT for reflecting tumors angiogenesis. Methods Fifty-four New Zealand white rabbits which weighted 2.5~3.0 kg were used in the experiment. Under general anesthesia, a needle was transthoracically inserted into the right lung, 0.5 ml viable VX2 tumor cell suspension was slowly injected through the needle to establish the model. 11C-choline and 18F-FDG PET-CT were performed after 10~11 d. The tumors SUVmax were calculated. The sections were stained with hematoxylin and eosin, and immunostained for CD34. Assessment of microvessel density (MVD) was performed by computer-assisted image analysis. The relationship 11C-choline SUVmax and 18F-FDG SUVmax with tumor size and MVD were statistically analyzed. Results Thirty-three rabbits successfully completed all imaging examinations. 11C-choline and 18F-FDG differently accumulated in all lung VX2 tumors. The mean of 11C-choline SUVmax was 4.02±3.07 (1.4~12.2), and the mean of 18F-FDG SUVmax was 5.70±3.45 (1.0~13.0). The mean size of tumor was(1.68±1.61)cm3 (0.13~8.00 cm3). Under high power microscope field of vision (200×, 0.739 mm2), the mean of MVD was 35.8±13.6(13~64). 11C-choline SUVmax did not correlate with tumor size and MVD. 18F-FDG SUVmax significantly and positively related to MVD (r=0.525, P=0.002). There was a critical positive correlation between 18F-FDG SUVmax and tumor size (r=0.335, P=0.057). Conclusions In the rabbit VX2 lung tumor model, 18F-FDG SUVmax correlated with MVD, so 18F-FDG PET-CT could reflect tumor angiogenesis. 11C-choline SUVmax did not statistically correlate with MVD, and 11C-choline PET-CT could not reflect tumor angiogenesis. -
Key words:
- Lung neoplasms /
- Positron-emission tomopraphy /
- Fluorodeoxyglucose F18 /
- 11C-choline /
- Model, animal /
- Rabbits /
- Microvessel density
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