生物发光成像的特点及应用

杨丽平; 赵敬湘; 裴雪涛

doi:10.3760/cma.j.issn.1673-4114.2009.04.018

生物发光成像的特点及应用

杨丽平 , 赵敬湘 , 裴雪涛

文章导航 > 国际放射医学核医学杂志 > 2009, 33 > 4: (249-252)

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生物发光成像的特点及应用

1. 北京军区总医院263临床部神经内科, 北京, 101149;
2. 中国人民解放军军事医学科学院输血研究所干细胞生物学研究室, 北京, 100850

通讯作者: 裴雪涛, peixt@nic.ac.cn

基金项目:
国家高技术研究发展计划(863计划)(2006AA2A107)

国家重点基础研究发展规划(973计划)(2005CB522702)

Bioluminescence imaging characteristics and application

1. Department of Neurology, NO. 263 Hospital, Beijing 101149, China;
2. Department of Stem Cell and Regenerative Medsine, Beijing Institute of Transfusion Medsine, Beijing 100850, China

Corresponding author: PEI Xue-tao, peixt@nic.ac.cn

摘要: 生物发光成像(BLI)是通过荧光素酶基因标记细胞或DNA,在ATP及氧气存在条件下,催化荧光素的氧化反应而发光,从而能够直接监控活体内的细胞活动和基因行为。该文通过比较BLI与MRI,PET、放射成像的异同,以及BLI在肿瘤、干细胞和免疫细胞运输、细胞凋亡等方面的应用,为更好地推广BLI的应用提供依据。
- 体层摄影术,光学 /
- 荧光素酶类 /
- 肿瘤 /
- 干细胞
Abstract: Bioluminescence imaging (BLI) by luciferase gene marked cells or DNA, in the presence of ATP and oxygen, catalytic oxidation reaction of fluorescein luminescence. So that it can directly monitor in vivo cell activity and gene behavior. In this paper, by comparing the BLI and MRI, PET, radiography of the similarities and differences, as well as about their cancer, stem cells and immune cells transportation apoptosis and other aspects of the application, in order to better provide the basis for promoting the application of BLI.
- Tomography,optical /
- Fluoresceins /
- Neoplasms /
- Stem cells
本作品遵循Signature-Non-Commercial Use 4.0 International (CC BY-NC 4.0)协议

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生物发光成像的特点及应用

通讯作者: 裴雪涛, peixt@nic.ac.cn

1. 北京军区总医院263临床部神经内科, 北京, 101149;
2. 中国人民解放军军事医学科学院输血研究所干细胞生物学研究室, 北京, 100850

收稿日期: 2009-04-22

基金项目: 国家高技术研究发展计划(863计划)(2006AA2A107)国家重点基础研究发展规划(973计划)(2005CB522702)

关键词:

摘要: 生物发光成像(BLI)是通过荧光素酶基因标记细胞或DNA,在ATP及氧气存在条件下,催化荧光素的氧化反应而发光,从而能够直接监控活体内的细胞活动和基因行为。该文通过比较BLI与MRI,PET、放射成像的异同,以及BLI在肿瘤、干细胞和免疫细胞运输、细胞凋亡等方面的应用,为更好地推广BLI的应用提供依据。